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Metabolomic evaluation of pulsed electric field-induced stress on potato tissue

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Abstract

Metabolite profiling was used to characterize stress responses of potato tissue subjected to reversible electroporation, providing insights on how potato tissue responds to a physical stimulus such as pulsed electric fields (PEF), which is an artificial stress. Wounded potato tissue was subjected to field strengths ranging from 200 to 400 V/cm, with a single rectangular pulse of 1 ms. Electroporation was demonstrated by propidium iodide staining of the cell nucleae. Metabolic profiling of data obtained through GC/TOF-MS and UPLC/TOF-MS complemented with orthogonal projections to latent structures clustering analysis showed that 24 h after the application of PEF, potato metabolism shows PEF-specific responses characterized by the changes in the hexose pool that may involve starch and ascorbic acid degradation.

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Abbreviations

ACN:

Acetonitrile

DRE:

Dynamic range enhancement

GC/TOF-MS:

Gas chromatography/time-of-flight mass spectrometry

H-MCR:

Hierarchical multivariate curve resolution

MST:

Mass spectral tag

MSTFA:

N-Methyl-N-(trimethylsilyl)trifluoroacetamide

NIST:

National Institute of Standards and Technology

OPLS:

Orthogonal projections to latent structures

PEF:

Pulsed electric field

RI:

Retention index

UPLC/TOF-MS:

Ultra performance liquid chromatography/time-of-flight mass spectrometry

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Acknowledgments

The authors are grateful to Prof. Susanne Widell from the Department of Cell and Organism Biology, Lund University, Sweden, for the use of microscopic facilities. This study was supported by grants from the Portuguese Foundation of Science (FCT, Portugal) and The Royal Physiographic Society in Lund, Sweden.

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Authors and Affiliations

  1. Centro de Engenharia Biológica, Institute for Biotechnology and Bioengineering (IBB), Universidade do Minho, Campus de Gualtar, 4710-057, Braga, Portugal

    Federico Gómez Galindo & António Vicente

  2. Department of Food Technology Engineering and Nutrition, Lund University, 124, 221 00, Lund, Sweden

    Petr Dejmek

  3. Department of Forest Genetics and Plant Physiology, Umeå Plant Science Center, Swedish University of Agricultural Sciences, 901 87, Umeå, Sweden

    Krister Lundgren & Thomas Moritz

  4. Department of Cell and Organism Biology, Lund University, Sölvegatan 35B, 223 62, Lund, Sweden

    Allan G. Rasmusson

Authors
  1. Federico Gómez Galindo
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  2. Petr Dejmek
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  3. Krister Lundgren
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  4. Allan G. Rasmusson
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  5. António Vicente
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  6. Thomas Moritz
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Correspondence to Federico Gómez Galindo.

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Galindo, F.G., Dejmek, P., Lundgren, K. et al. Metabolomic evaluation of pulsed electric field-induced stress on potato tissue. Planta 230, 469–479 (2009). https://doi.org/10.1007/s00425-009-0950-2

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