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PbrPOE21 inhibits pear pollen tube growth in vitro by altering apical reactive oxygen species content

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Abstract

Main conclusion

Genome-wide identification, tissue-specific expression analysis and functional characterization of selected genes containing the pear Pollen Olea europaea I domain reveal their roles in pollen tube growth.

Abstract

Genes containing the Pollen Olea europaea I (POE) domain play crucial roles in diverse growth and developmental processes. Nevertheless, the specific functions of POE family members in progression of pollen tube growth (PTG) remain uncharacterized. We identified 45 PbrPOE genes in the pear (Pyrus bretschneideri) genome, clustered into seven subclasses. PbrPOE genes contained 1 to 11 exons and 0 to 10 introns, with exon/intron structure mostly conserved within each subclass. Whole-genome duplication has mainly contributed to the duplication pattern of PbrPOE genes in pear. Expression profiles of 45 PbrPOE genes in 12 different pear tissues revealed that six PbrPOE genes (PbrPOE6, 12, 21, 29, 35 and 41) of subclass B were highly expressed during the growth of the pear pollen tube in vitro. PbrPOE21 was selected for further functional analysis on the basis of its high and differential expression pattern in pollen. Antisense oligodeoxynucleotide assays demonstrated that PTG was augmented in vitro when PbrPOE21 expression was significantly inhibited. Moreover, pollen tube length in vitro was reduced when PbrPOE21 was transitorily over-expressed using particle bombardment technology. Exogenous PbrPOE21 recombinant protein inhibited PTG in vitro at an optimum concentration of 1.8 µM. PbrPOE21 also affected reactive oxygen species content in the pear pollen tube apex. We suggest that PbrPOE21 inhibits PTG in vitro by altering apical reactive oxygen species content.

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Abbreviations

POE:

Pollen Olea europaea I

as-ODN:

Antisense oligodeoxynucleotide

ROS:

Reactive oxygen species

SI:

Self-incompatibility

PTG:

Pollen tube growth

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Acknowledgements

We want to thank Huijun Jiao for bioinformatics guidance; Dongqing Shi for qPCR and protein instructions; and Luting Jia for providing selfless assistance. In addition, we still wish to express our thanks to Peng Wang and Zhihua Guo for English language correction. This study was supported by the National Key Research and Development Program of China (2018YFD1000107).

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  1. Ming Qian and Linlin Xu have contributed equally to this work

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  1. College of Horticulture, State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing, 210095, China

    Ming Qian, Linlin Xu, Chao Tang, Hao Zhang, Hongru Gao, Peng Cao, Hao Yin, Lei Wu, Juyou Wu, Chao Gu & Shaoling Zhang

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Qian, M., Xu, L., Tang, C. et al. PbrPOE21 inhibits pear pollen tube growth in vitro by altering apical reactive oxygen species content. Planta 252, 43 (2020). https://doi.org/10.1007/s00425-020-03446-7

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