Transcriptome and plant hormone analyses provide new insight into the molecular regulatory networks underlying hybrid lethality in cabbage (Brassica oleracea)

Xiao, Zhiliang; Liu, Xing; Fang, Zhiyuan; Yang, Limei; Zhang, Yangyong; Wang, Yong; Zhuang, Mu; Lv, Honghao

doi:10.1007/s00425-021-03608-1

Transcriptome and plant hormone analyses provide new insight into the molecular regulatory networks underlying hybrid lethality in cabbage (Brassica oleracea)

Original Article
Published: 11 April 2021

Volume 253, article number 96, (2021)
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Zhiliang Xiao¹,
Xing Liu¹,
Zhiyuan Fang¹,
Limei Yang¹,
Yangyong Zhang¹,
Yong Wang¹,
Mu Zhuang¹ &
…
Honghao Lv ORCID: orcid.org/0000-0003-2635-3042¹

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Abstract

Main conclusion

Comparative morphological, transcriptomic and phytohormone analyses reveal a defence network leading to PCD involved in cabbage hybrid lethality.

Abstract

Hybrid lethality (HL) plays an essential role in the stability of a population by blocking gene exchange between species, but the molecular mechanism remains largely undetermined. In this study, we performed phenotype, transcriptome and plant hormone analyses of HL in cabbage. Phenotype analysis confirmed that HL is characterised by a typical programmed cell death (PCD) process. A time-resolved RNA-Seq identified 2724 differentially expressed genes (DEGs), and functional annotations analyses revealed that HL was closely associated with the defence response. A defence regulation network was constructed based on the plant–pathogen interaction pathway and MAPK signalling pathway, which comprised DEGs related to Ca²⁺ and hydrogen peroxide (H₂O₂) leading to PCD. Moreover, important DEGs involved in hormone signal transduction pathways including salicylic acid (SA) and jasmonic acid (JA) were identified, which were further confirmed by endogenous and exogenous SA and JA measurements. Our results identified key genes and pathways in the regulating network of HL in cabbage, and might open the gate for revealing the molecular mechanism of HL in plants.

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Abbreviations

DEG:: Differentially expressed gene
HL:: Hybrid lethality
HR:: Hypersensitive response
JA:: Jasmonic acid
PCD:: Programmed cell death
SA:: Salicylic acid
TF:: Transcription factor

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Acknowledgements

The work reported here was performed at the Key Laboratory of Biology and Genetic Improvement of Horticultural Crops, Ministry of Agriculture, Beijing 100081, China.

Funding

This work was financially supported by grants from the National Natural Science Foundation of China (31572139), the Key Projects of the National Key Research and Development Program of China (2016YFD0100307), the Central Public-interest Scientific Institution Basal Research Fund (Y2020PT01), and the Science and Technology Innovation Program of the Chinese Academy of Agricultural Sciences (CAAS-ASTIP-IVFCAAS).

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Authors and Affiliations

Key Laboratory of Biology and Genetic Improvement of Horticultural Crops, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Ministry of Agriculture, 12# Zhongguancun Nandajie Street, Beijing, 100081, China
Zhiliang Xiao, Xing Liu, Zhiyuan Fang, Limei Yang, Yangyong Zhang, Yong Wang, Mu Zhuang & Honghao Lv

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Zhiliang Xiao
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Xing Liu
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Zhiyuan Fang
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Limei Yang
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Yangyong Zhang
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Yong Wang
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Mu Zhuang
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Honghao Lv
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Correspondence to Mu Zhuang or Honghao Lv.

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Xiao, Z., Liu, X., Fang, Z. et al. Transcriptome and plant hormone analyses provide new insight into the molecular regulatory networks underlying hybrid lethality in cabbage (Brassica oleracea). Planta 253, 96 (2021). https://doi.org/10.1007/s00425-021-03608-1

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Received: 08 December 2020
Accepted: 24 March 2021
Published: 11 April 2021
DOI: https://doi.org/10.1007/s00425-021-03608-1

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Transcriptome and plant hormone analyses provide new insight into the molecular regulatory networks underlying hybrid lethality in cabbage (Brassica oleracea)