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Isolation and characterization of class II myosin genes from Haemonchus contortus

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An Erratum to this article was published on 25 March 2006

Abstract

In this study, cDNAs encoding myosin from the parasitic nematode Haemonchus contortus were isolated and characterized. Several exhibited a considerable degree of sequence variation at the nucleotide and limited divergence at the amino acid levels within the various functional domains. The results suggest that the cDNAs isolated represented a single myosin heavy chain, which, by comparison with a number of other myosins, is inferred to represent a homologue of a muscle myosin (CeMHCA) of the free-living nematode Caenorhabditis elegans. The findings could have implications for investigating cytoskeletal dynamics and/or signalling pathways.

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Acknowledgements

The authors would like to thank Michael Georgesz for the propagation of Caenorhabditis elegans. This work was supported by Novartis Animal Health Australasia Pty. Ltd., the Department of Primary Industries (DPI), Victoria, Australia, and the Australian Research Council (LP0346983 and LP0667795 to RBG). SN was the recipient of a postgraduate scholarship from the University of Melbourne and a “top-up award” from Novartis Animal Health.

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Authors and Affiliations

  1. Primary Industries Research Victoria, 475 Mickleham Road, Attwood, VIC, 3049, Australia

    M. Hu, D. Hartman & P. J. A. Presidente

  2. Department of Veterinary Science, The University of Melbourne, 250 Princes Highway, Werribee, VIC, 3030, Australia

    M. Hu & R. B. Gasser

  3. Biotechnology Research Institute, Macquarie University, Sydney, NSW, 2109, Australia

    R. B. Gasser

  4. Department of Biology, University of Saskatchewan, 112 Science Place, Saskatoon, SK, S7N 5EZ, Canada

    N. B. Chilton

  5. Moredun Research Institute, Pentlands Science Park, Bush Loan, Penicuik, Midlothian, Scotland, EH26 0PZ, UK

    A. J. Nisbet

Authors
  1. M. Hu
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  2. N. B. Chilton
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  3. D. Hartman
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  4. A. J. Nisbet
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  5. P. J. A. Presidente
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  6. R. B. Gasser
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Corresponding author

Correspondence to R. B. Gasser.

Additional information

Nucleotide sequence data reported in this paper are available in the GenBank, EMBL and DDBJ databases under the accession nos. DQ310759–DQ310761.

An erratum to this article can be found at http://dx.doi.org/10.1007/s00436-006-0188-x

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Hu, M., Chilton, N.B., Hartman, D. et al. Isolation and characterization of class II myosin genes from Haemonchus contortus . Parasitol Res 99, 200–203 (2006). https://doi.org/10.1007/s00436-006-0129-8

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  • DOI: https://doi.org/10.1007/s00436-006-0129-8

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