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Effectiveness of Zanthoxylum piperitum-derived essential oil as an alternative repellent under laboratory and field applications

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Abstract

Recently, there were considerable efforts made to promote the use of environmentally friendly and biodegradable natural insecticides and repellents, particularly from botanical sources. In this study, Zanthoxylum piperitum-derived essential oil isolated by steam distillation was investigated and compared to the standard synthetic repellent, N,N-diethyl-3-methylbenzamide (DEET), for repellency against mosquitoes under laboratory and field conditions. The oil of Z. piperitum alone and also with 5% vanillin added repelled laboratory-reared female Aedes aegypti with the median protection times of 1.5 and 2.5 h, respectively. These repellency times were proven to be significantly lower than those of DEET-based products: 25% DEET and 25% DEET + 5% vanillin demonstrating 3.5 and 5.5 h, respectively. However, when applied under field conditions, Z. piperitum oil + 5% vanillin was found to provide better protection against a wide range of natural mosquito populations than 25% DEET + 5% vanillin. While the product of DEET was effective in reducing bites with 99.7% protection, the protective effect of Z. piperitum oil product appeared complete (100%). It had a protective effect against Aedes gardnerii, Anopheles barbirostris, Armigeres subalbatus, Culex tritaeniorhynchus, Culex gelidus, Culex vishnui group, and Mansonia uniformis. The better repellency against a wide range of field mosquitoes derived from Z. piperitum oil products suggested an advantage of efficacy by providing a broad spectrum of activity. Therefore, Z. piperitum could have potential for use in the development of combined repellents as a natural active ingredient, synergist, or additive to conventional synthetic chemicals, particularly in situations when DEET is ineffective and impractical.

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References

  • Amer A, Mehlhorn H (2006a) Repellency effect of forty-one essential oils against Aedes, Anopheles, and Culex mosquitoes. Parasitol Res (in press). DOI 10.1007/s00436-006-0184-1

  • Amer A, Mehlhorn H (2006b) The sensilla of Aedes and Anopheles mosquitoes and their importance in repellency. Parasitol Res (in press). DOI 10.1007/s00436-006-0185-0

  • Ansari MA, Razdan RK (1995) Relative efficacy of various oils in repelling mosquitoes. Indian J Malariol 32:104–111

    PubMed  CAS  Google Scholar 

  • Ansari MA, Vasudevan P, Tandon M, Razdan RK (2000) Larvicidal and mosquito repellent action of peppermint (Mentha piperita) oil. Bioresour Technol 71:267–271

    Article  CAS  Google Scholar 

  • Barnard DR (2000) Repellents and toxicants for personal protection. In: World Health Organization, Department of Control, Prevention and Eradication, Programme on Communicable Diseases, WHO Pesticide Evaluation Scheme (WHOPES), WHO/CDS/WHOPES/GCDPP/2000.5, WHO, Geneva, Switzerland

  • Barnard DR, Xue RD (2004) Laboratory evaluation of mosquito repellents against Aedes albopictus, Culex nigripalpus, and Ochierotatus triseriatus (Diptera: Culicidae). J Med Entomol 41:726–730

    Article  PubMed  CAS  Google Scholar 

  • Browne LB (1997) Host-related responses and their suppression: some behavioral considerations. In: Shorey HH, McKelvey JJ Jr (eds) Chemical control of insect behavior. Wiley, New York, pp 117–127

    Google Scholar 

  • Chaithong U, Kamsuk K, Choochote W, Jitpakdi A, Tippawangkosol P, Tuetun B, Champakaew D, Pitasawat B (2006) Chemical components and repellent activity of selected essential oils against Aedes aegypti (Diptera: Culicidae). Fitoterapia (In press)

  • Collins DA, Brady JN (1993) Assessment of the efficacy of quwenling as a mosquito repellent. Phytother Res 7:17–20

    Article  CAS  Google Scholar 

  • Das NG, Nath DR, Baruah I, Talukdar PK, Das SC (1999) Field evaluation of herbal mosquito repellents. J Commun Dis 31:241–245

    PubMed  CAS  Google Scholar 

  • Das NG, Baruah I, Talukdar PK, Das SC (2003) Evaluation of botanicals as repellents against mosquitoes. J Vector Borne Dis 40:49–53

    PubMed  CAS  Google Scholar 

  • Dua VK, Gupta NC, Pandey AC, Sharma VP (1996) Repellency of Lantana camara (Verbenaceae) flowers against Aedes mosquitoes. J Am Mosq Control Assoc 12:406–408

    PubMed  CAS  Google Scholar 

  • Fradin MS (1998) Mosquitoes and mosquito repellents: a clinician’s guide. Ann Intern Med 128:931–940

    PubMed  CAS  Google Scholar 

  • Fradin MS, Day JF (2002) Comparative efficacy of insect repellents against mosquito bites. New Engl J Med 347:13–18

    Article  PubMed  CAS  Google Scholar 

  • Frances SP, Eikarat N, Sripongsai B, Eamsila C (1993) Response of Anopheles dirus and Aedes albopictus to repellents in the laboratory. J Am Mosq Control Assoc 9:474–476

    PubMed  CAS  Google Scholar 

  • Frances SP, Cooper RD, Popat S, Beebe NW (2001) Field evaluation of repellents containing deet and AI3-37220 against Anopheles koliensis in Papua New Guinea. J Am Mosq Control Assoc 17:42–44

    PubMed  CAS  Google Scholar 

  • Frances SP, Waterson DGE, Beebe NW, Cooper RD (2004) Field evaluation of repellent formulations containing deet and picaridin against mosquitoes in Northern Territory, Australia. J Med Entomol 41:414–417

    Article  PubMed  CAS  Google Scholar 

  • Golenda CF, Solberg VB, Burge R, Gambel JM, Wirtz RA (1999) Gender-related efficacy difference to an extended duration formulation of topical N,N-diethyl-m-toluamide (DEET). Am J Trop Med Hyg 60:654–657

    PubMed  CAS  Google Scholar 

  • Govere J, Durrheim DN, Baker L, Hunt R, Coetzee M (2000) Efficacy of three insect repellents against the malaria vector Anopheles arabiensis. Med Vet Entomol 14:441–444

    Article  PubMed  CAS  Google Scholar 

  • Gupta RK, Rutledge LC (1989) Laboratory evaluation of controlled release repellent formulations on human volunteers under three climate regimens. J Am Mosq Control Assoc 5:52–55

    PubMed  CAS  Google Scholar 

  • Gupta RK, Rutledge LC (1991) Controlled release repellent formulations on human volunteers under three climate regimens. J Am Mosq Control Assoc 7:490–493

    PubMed  CAS  Google Scholar 

  • Hadfield-Law L (2000) Head lice for A & E nurses. Accid Emerg Nurs 8:84–87

    Article  PubMed  CAS  Google Scholar 

  • Isman MB (1999) Pesticides based on plant essential oils. Pestic Outlook 10:68–72

    CAS  Google Scholar 

  • Limsuwan S, Rongsriyam Y, Kerdpibule V, Apiwathnasorn C, Chiang GL, Cheong WH (1987) Rearing Techniques for Mosquitoes. In: Sucharit S, Supavej S (eds) Practical Entomology. Malaria and filariasis, 1st edn. Museum and Reference Centre, Faculty of Tropical Medicine, Mahidol University, Bangkok

    Google Scholar 

  • Matsuda BM, Surgeoner GA, Heal JD, Tucker AO, Maciarello MJ (1996) Essential oil analysis and field evaluation of the citrosa plant “Pelargonium citrosum” as a repellent against populations of Aedes mosquitoes. J Am Mosq Control Assoc 12:69–74

    PubMed  CAS  Google Scholar 

  • Mumcuoglu KY, Miller J, Zamir C, Zentner G, Helbin V, Ingber A (2002) The in vivo pediculicidal efficacy of a natural remedy. Isr Med Assoc J 4:790–793

    PubMed  Google Scholar 

  • Nentwig G (2003) Use of repellents as prophylactic agents. Parasitol Res 90:S40–S48

    PubMed  Google Scholar 

  • Omolo MO, Okinyo D, Ndiege IO, Lwande W, Hassanali A (2004) Repellency of essential oils of some Kenyan plants against Anopheles gambiae. Phytochemistry 65:2797–2802

    Article  PubMed  CAS  Google Scholar 

  • Osimitz TG, Grothaus RH (1995) The present safety assessment of deet. J Am Mosq Control Assoc 11:274–278

    PubMed  CAS  Google Scholar 

  • Oyedele AO, Gbolade AA, Sosan MB, Adewoyin FB, Soyelu OL, Orafidiya OO (2002) Formulation of an effective mosquito-repellent topical product from lemongrass oil. Phytomedicine 9:259–262

    Article  PubMed  CAS  Google Scholar 

  • Pitasawat B, Choochote W, Tuetun B, Tippawangkosol P, Kanjanapothi D, Jitpakdi A, Riyong D (2003) Repellency of aromatic turmeric Curcuma aromatica under laboratory and field conditions. J Vector Ecol 28:234–240

    PubMed  Google Scholar 

  • Rattanarithikul R, Panthusiri P (1994) An illustrated key to the medically important mosquitoes of Thailand. US Army Medica; Companent, Southeast Asia Treaty Organization, Bangkok

    Google Scholar 

  • Rozendaal JA (1997) Vector control, methods for use by individuals and communities. World Health Organization, Geneva, Switzerland, pp 7–177

    Google Scholar 

  • Schreck CE (1985) The status of deet (N,N diethyl-m-toluamide) as a repellent for Anopheles albimanus. J Am Mosq Control Assoc 1:98–100

    PubMed  CAS  Google Scholar 

  • Sharma VP, Ansari MA (1994) Personal protection from mosquitoes (Diptera: Culicidae) by burning neem oil in Kerosene. J Med Entomol 31:505–507

    PubMed  CAS  Google Scholar 

  • Sharma SK, Dua VK, Sharma VP (1995) Field study on the mosquito repellent action of neem oil. Southeast Asian J Trop Med Public Health 26:180–182

    PubMed  CAS  Google Scholar 

  • Stuart AE, Estambale BA (2003) The repellent and antifeedant activity of Myrica gale oil against Aedes aegypti mosquitoes and its enhancement by the addition of salicyluric acid. J R Coll Physicians Edinb 33:209–214

    Google Scholar 

  • Tanaka K, Mizusawa K, Saugstad E (1979) Mosquitoes of Japan and Korea. Contrib Am Entomol Inst 16:987

    Google Scholar 

  • Tawatsin A, Wratten SD, Scott RR, Thavara U, Techadamrongsin Y (2001) Repellency of volatile oils from plants against three mosquito vectors. J Vector Ecol 26:76–82

    PubMed  CAS  Google Scholar 

  • Tuetun B, Choochote W, Kanjanapothi D, Rattanachanpichai E, Chaithong U, Chaiwong P, Jitpakdi A, Tippawangkosol P, Riyong D, Pitasawat B (2005) Repellent properties of celery, Apium graveolens L., compared with commercial repellents, against mosquitoes under laboratory and field conditions. Trop Med Int Health 10:1190–1198

    Article  PubMed  Google Scholar 

  • WHO (1996) Report of the WHO informal consultation on the evaluation and testing of insecticides, CTD/WHOPES/IC/96.1. Control of Tropical Diseases, World Health Organization, Geneva

    Google Scholar 

  • Yap HH, Jahangir K, Chong ASC, Adanan CR, Chong NL, Malik YA, Rohaizat B (1998) Field efficacy of a new repellent, KBR 3023, against Aedes albopictus (Skuse) and Culex quinquefasciatus (Say) in a tropical environment. J Vector Ecol 23:62–68

    PubMed  CAS  Google Scholar 

  • Zhu BCR, Henderson G, Chen F, Fei H, Laine RA (2001) Evaluation of vetiver oil and seven insect-active essential oils against the Formosan subterranean termite. J Chem Ecol 27:1617–1625

    Article  PubMed  CAS  Google Scholar 

Download references

Acknowledgements

This work received financial support from the Faculty of Medicine Research Fund, Faculty of Medicine, Chiang Mai University, Chiang Mai Province, Thailand. The authors would like to thank the individuals who served as subject volunteers and also the staff members of the Department of Parasitology, Faculty of Medicine, Chiang Mai University for their cooperation.

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Authors and Affiliations

  1. Department of Parasitology, Faculty of Medicine, Chiang Mai University, Chiang Mai, 50200, Thailand

    K. Kamsuk, W. Choochote, U. Chaithong, A. Jitpakdi, P. Tippawangkosol, D. Riyong & B. Pitasawat

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  1. K. Kamsuk
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  2. W. Choochote
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  3. U. Chaithong
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  4. A. Jitpakdi
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  5. P. Tippawangkosol
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  6. D. Riyong
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  7. B. Pitasawat
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Correspondence to B. Pitasawat.

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Kamsuk, K., Choochote, W., Chaithong, U. et al. Effectiveness of Zanthoxylum piperitum-derived essential oil as an alternative repellent under laboratory and field applications. Parasitol Res 100, 339–345 (2007). https://doi.org/10.1007/s00436-006-0256-2

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