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Anti-parasitic activities of specific bacterial extracellular products of Streptomyces griseus SDX-4 against Ichthyophthirius multifiliis

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Abstract

The ciliate Ichthyophthirius multifiliis is one of the most pathogenic parasites of fish maintained in captivity. In this study, effects of bacterial extracellular products of Streptomyces griseus SDX-4 against I. multifiliis were determined. The fermentation liquor of S. griseus was extracted successively in a separating funnel with petroleum ether, ethyl acetate, and n-butanol. In vitro assays revealed that the n-butanol extracts (NBu-E) and ethyl acetate extracts (Eto-E) of S. griseus were observed to be more effective against theronts than the other extracts with median effective concentration (EC50) values of 0.86 and 12.5 mg L−1, respectively, and significantly reduced the survival of the tomonts and the total number of theronts released by the tomonts (P < 0.05). All encysted tomonts were killed when the concentration of NBu-E was 30.0 mg L−1. Results of in vivo test demonstrated that the number of I. multifiliis trophonts on the grass carp treated with NBu-E was markedly lower compared to the control group at 11 days after exposed to theronts (P < 0.05). In the control group, 100 % mortality was observed owing to heavy I. multifiliis infection at 11 days after the exposure. On the other hand, only 9.5 % mortality owing to parasite infection was recorded in the groups treated with the NBu-E (30 mg L−1). The median lethal dose (LD50) of NBu-E for grass carp was 152.4 mg L−1. Our results indicate that n-butanol extract of S. griseus will be useful in aquaculture for controlling I. multifiliis infections.

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Acknowledgments

The research was supported by the National Natural Science Foundation of China (31302211), the Key Laboratory of East China Sea and Oceanic Fishery Resources Exploitation and Utilization (K201209), and the key project program of Huzhou City (2012GN12).

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Authors and Affiliations

  1. Zhejiang Institute of Freshwater Fisheries, Huzhou, Zhejiang, 313001, China

    Jia-Yun Yao, Wen-Lin Yin, Yang Xu & Jin-Yu Shen

  2. East China Sea Fisheries Research Institute, Shanghai, 200090, China

    Xin-Cang Li

  3. National Fisheries Technology Extension Center, Beijing, 100125, China

    Gang Li

  4. Wenzhou Medical University, Wenzhou, Zhejiang, 325035, China

    Wei-Ming Ai

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  1. Jia-Yun Yao
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  2. Wen-Lin Yin
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  3. Xin-Cang Li
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  4. Gang Li
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  5. Yang Xu
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  6. Wei-Ming Ai
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Correspondence to Jin-Yu Shen.

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Yao, JY., Yin, WL., Li, XC. et al. Anti-parasitic activities of specific bacterial extracellular products of Streptomyces griseus SDX-4 against Ichthyophthirius multifiliis . Parasitol Res 113, 3111–3117 (2014). https://doi.org/10.1007/s00436-014-4020-8

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  • DOI: https://doi.org/10.1007/s00436-014-4020-8

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