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Production of anti-SAG1 IgY antibody against Toxoplasma gondii parasites and evaluation of antibody activity by ELISA method

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Abstract

Chicken egg yolk antibody, also known as immunoglobulin Y (IgY), is the predominant class of serum immunoglobulin in birds. IgY has many advantages over mammalian antibodies, such as enhanced immunogenicity conserved mammalian proteins exhibited in birds due to their phylogenetic distance, non-invasive rapid, and economical collection system. However, there are limited studies about IgY production against Toxoplasma, which is a worldwide veterinary and public health problem. In this study, the production of specific IgY antibodies against the surface antigen 1 (SAG1) protein of Toxoplasma gondii and the determination of antibody activity via the enzyme-linked immunosorbent assay (ELISA) method were conducted. According to ELISA, Western blot, and NanoDrop results, specific and higher amounts of IgY antibody against SAG1 were obtained with this study. Considering the advantages of IgY and importance of SAG1 for the diagnosis of toxoplasmosis, it is expected that anti-SAG1 IgY will play an increasing role and gain commercial value in research, diagnostics, and immunotherapy against toxoplasmosis in the future.

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Acknowledgments

The authors thank the Yildiz Technical University Scientific Research Projects Coordination Department (YTU BAP, Project Number: 2015-07-04-GEP03).

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Authors and Affiliations

  1. Department of Bioengineering, Faculty of Chemical and Metallurgical Engineering, Yildiz Technical University, 34210, Esenler, Istanbul, Turkey

    Rabia Cakir-Koc

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  1. Rabia Cakir-Koc
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Correspondence to Rabia Cakir-Koc.

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All of the procedures used in the trial were approved by the animal ethics committees of the University of Osmangazi, Eskisehir, Turkey.

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Cakir-Koc, R. Production of anti-SAG1 IgY antibody against Toxoplasma gondii parasites and evaluation of antibody activity by ELISA method. Parasitol Res 115, 2947–2952 (2016). https://doi.org/10.1007/s00436-016-5047-9

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  • DOI: https://doi.org/10.1007/s00436-016-5047-9

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