Abstract
Canine vector-borne pathogens are a group of widespread microorganisms and nematodes transmitted by arthropods that do not only impact dog health but may also pose a health risk to humans as many of them are zoonotic. As no data exist on the presence of canine vector-borne pathogens in Kosovo, we here present a first study on the seroprevalence of selected vector-borne pathogens, the dog heartworm Dirofilaria immitis and the bacteria Anaplasma spp., Ehrlichia spp. and Borrelia burgdorferi sensu lato. The study was carried out from July to October 2019 in all regions of Kosovo and included 149 clinically healthy dogs (84 owned, 40 sheltered and 25 free-ranging). Blood samples collected from each individual were tested using a commercially available rapid multiplex enzyme-linked immunosorbent assay. In total, 37.6% of the examined dogs were seropositive for one or more of the target pathogens. Most frequently, antibodies were found against Anaplasma spp. (24.8% of the dogs tested), followed by antigen detection of D. immitis (14.8%) and antibodies of B. burgdorferi s.l. (1.3%). The lowest antibody prevalence (0.7%) showed E. canis where only one dog was found positive. This preliminary study demonstrates the circulation of several zoonotic vector-borne pathogens in natural cycles involving dogs in Kosovo. It should trigger studies on infection prevalences in humans and initiate vector surveillance programmes in order to manage and control transmission and the diseases associated with the pathogens.
References
Aguirre E, Sainz A, Dunner S, Amusategui I, Lopez L, Rodriguez-Franco F, Luaces I, Cortes O, Tesouro MA (2004) First isolation and molecular characterization of Ehrlichia canis in Spain. Vet Parasitol 125(3–4):365–372. https://doi.org/10.1016/j.vetpar.2004.08.007
Angelou A, Gelasakis AI, Verde N, Pantchev N, Schaper R, Chandrashekar R, Papadopoulos E (2019) Prevalence and risk factors for selected canine vector-borne diseases in Greece. Parasit Vectors 12:283. https://doi.org/10.1186/s13071-019-3543-3
Beugnet F, Chalvet-Monfray K (2013) Impact of climate change in the epidemiology of vector-borne diseases in domestic carnivores. Comp Immunol Microbiol Infect Dis 36:559–566. https://doi.org/10.1016/j.cimid.2013.07.003
Beugnet F, Marie JL (2009) Emerging arthropod-borne diseases of companion animals in Europe. Vet Parasitol 163:298–305. https://doi.org/10.1016/j.vetpar.2009.03.028
Dantas-Torres F, Chomel BB, Otranto D (2012) Ticks and tick-borne diseases: a one health perspective. Trends Parasitol 28:437–446. https://doi.org/10.1016/j.pt.2012.07.003
Day MJ (2011) The immunopathology of canine vector-borne diseases. Parasit Vectors 4:48. https://doi.org/10.1186/1756-3305-4-48
Hamel D, Silaghi C, Knaus M, Visser M, Kusi I, Rapti D, Rehbein S, Pfister K (2009) Detection of Babesia canis subspecies and other arthropod-borne diseases in dogs from Tirana, Albania. Wien Klin Wochenschr 121:42–45. https://doi.org/10.1007/s00508-009-1234-3
Hamel D, Shukullari E, Rapti D, Silaghi C, Pfister K, Rehbein S (2016) Parasites and vector-borne pathogens in client-owned dogs in Albania. Blood pathogens and seroprevalence of parasitic and other infectious agents. Parasitol Res 115:489–499. https://doi.org/10.1007/s00436-015-4765-8
Hubálek Z (2009) Epidemiology of Lyme borreliosis. Curr Probl Dermatol 37:31–50. https://doi.org/10.1159/000213069
Jurković D, Beck A, Huber D, Mihaljević Ž, Polginhorne A, Martinković F, Lukacević D, Pilat M, Brezak R, Bosnić S, Beck R (2019) Seroprevalence of vector-borne pathogens in dogs from Croatia. Parasitol Res 118:347–352. https://doi.org/10.1007/s00436-018-6129-7
Kosovo Environmental Protection Agency. Report of the state of nature 2008–2009 (2010). Available from: http://www.ammk-rks.net/repository/docs/Raporti_Natyra_Eng.pdf. accessed 20 May 2020
Lazri T, Duscher G, Edelhofer R, Bytyci B, Gjino P, Joachim A (2008) Arthropod-borne parasites of dogs, especially Leishmania, in the Kosovo and Albania. Wien Klin Wochenschr 120:54–5810. https://doi.org/10.1007/s00508-008-1076-4
Lozano R, Naghavi M, Foreman K, Lim S, Shibuya K, Aboyans V, Abraham J, Adair T, Aggarwal R, Ahn SY, AlMazroa MA, Alvarado M, Anderson HR, Anderson LM, Andrews KG, Atkinson C, Baddour LM, Barker-Collo S, Bartels DH, Bell ML, Benjamin EJ, Bennett D, Bhalla K, Bikbov B, Abdulhak AB, Birbeck G, Blyth F, Bolliger I, Boufous S, Bucello C, Burch M, Burney P, Carapetis J, Chen H, Chou D, Chugh SS, Coffeng LE, Colan SD, Colquhoun S, Colson KE, Condon J, Connor MD, Cooper LT, Corriere M, Cortinovis M, de Vaccaro KC, Couser W, Cowie BC, Criqui MH, Cross M, Dabhadkar KC, Dahodwala N, de Leo D, Degenhardt L, Delossantos A, Denenberg J, Des Jarlais DC, Dharmaratne SD, Dorsey ER, Driscoll T, Duber H, Ebel B, Erwin PJ, Espindola P, Ezzati M, Feigin V, Flaxman AD, Forouzanfar MH, Fowkes FGR, Franklin R, Fransen M, Freeman MK, Gabriel SE, Gakidou E, Gaspari F, Gillum RF, Gonzalez-Medina D, Halasa YA, Haring D, Harrison JE, Havmoeller R, Hay RJ, Hoen B, Hotez PJ, Hoy D, Jacobsen KH, James SL, Jasrasaria R, Jayaraman S, Johns N, Karthikeyan G, Kassebaum N, Keren A, Khoo JP, Knowlton LM, Kobusingye O, Koranteng A, Krishnamurthi R, Lipnick M, Lipshultz SE, Ohno SL, Mabweijano J, MacIntyre MF, Mallinger L, March L, Marks GB, Marks R, Matsumori A, Matzopoulos R, Mayosi BM, McAnulty JH, McDermott MM, McGrath J, Memish ZA, Mensah GA, Merriman TR, Michaud C, Miller M, Miller TR, Mock C, Mocumbi AO, Mokdad AA, Moran A, Mulholland K, Nair MN, Naldi L, Narayan KMV, Nasseri K, Norman P, O'Donnell M, Omer SB, Ortblad K, Osborne R, Ozgediz D, Pahari B, Pandian JD, Rivero AP, Padilla RP, Perez-Ruiz F, Perico N, Phillips D, Pierce K, Pope CA III, Porrini E, Pourmalek F, Raju M, Ranganathan D, Rehm JT, Rein DB, Remuzzi G, Rivara FP, Roberts T, de León FR, Rosenfeld LC, Rushton L, Sacco RL, Salomon JA, Sampson U, Sanman E, Schwebel DC, Segui-Gomez M, Shepard DS, Singh D, Singleton J, Sliwa K, Smith E, Steer A, Taylor JA, Thomas B, Tleyjeh IM, Towbin JA, Truelsen T, Undurraga EA, Venketasubramanian N, Vijayakumar L, Vos T, Wagner GR, Wang M, Wang W, Watt K, Weinstock MA, Weintraub R, Wilkinson JD, Woolf AD, Wulf S, Yeh PH, Yip P, Zabetian A, Zheng ZJ, Lopez AD, Murray CJL (2012) Global and regional mortality from 235 causes of death for 20 age groups in 1990 and 2010: a systematic analysis for the Global Burden of Disease Study 2010. Lancet 380:2095–2128. https://doi.org/10.1016/S0140-6736(12)61728-0
Maggi RG, Birkenheuer AJ, Hegarty BC, Bradley JM, Levy MG, Breitschwerdt EB (2014) Comparison of serological and molecular panels for diagnosis of vector-borne diseases in dogs. Parasit Vectors 7:12. https://doi.org/10.1186/1756-3305-7-127
Mircean V, Dumitrache MO, Györke A, Pantchev N, Jodies R, Mihalca AD, Cozma V (2012) Seroprevalence and geographic distribution of Dirofilaria immitis and tick-borne infections (Anaplasma phagocytophilum, Borrelia burgdorferi sensu lato and Ehrlichia canis) in dogs from Romania. Vector- Borne Zoonotic Dis 12:595–604. https://doi.org/10.1089/vbz.2011.0915
Montenegro VM, Bonilla MC, Kaminsky D, Romero-Zúñiga JJ, Siebert S, Krämer F (2017) Serological detection of antibodies to Anaplasma spp., Borrelia burgdorferi sensu lato and Ehrlichia canis and of Dirofilaria immitis antigen in dogs from Costa Rica. Vet Parasitol 236:97–107. https://doi.org/10.1016/j.vetpar.2017.02.009
Muja-Bajraktari N, Zhushi-Etemi F, Dikolli-Velo E, Kadriaj P, Gunay F (2019) The composition, diversity, and distribution of mosquito fauna (Diptera: Culicidae) in Kosovo. J Vector Ecol 44(1):94–104. https://doi.org/10.1111/jvec.12333
Nicholson WL, Allen KE, McQuiston JH, Breitschwerdt EB, Little SE (2010) The increasing recognition of rickettsial pathogens in dogs and people. Trends Parasitol 26:205–212. https://doi.org/10.1016/j.pt.2010.01.007
Otranto D, Dantas-Torres F, Breitschwerdt EB (2009a) Managing canine vector borne diseases of zoonotic concern: part one. Trends Parasitol 25:157–163. https://doi.org/10.1016/j.pt.2009.01.003
Otranto D, Dantas-Torres F, Breitschwerdt EB (2009b) Managing canine vector-borne diseases of zoonotic concern: part two. Trends Parasitol 25:228–235. https://doi.org/10.1016/j.pt.2009.02.005
Pantchev N, Schnyder M, Vrhovec MG, Schaper R, Tsachev I (2015) Current surveys of the seroprevalence of Borrelia burgdorferi, Ehrlichia canis, Anaplasma phagocytophilum, Leishmania infantum, Babesia canis, Angiostrongylus vasorum and Dirofilaria immitis in dogs in Bulgaria. Parasitol Res 114:117–130. https://doi.org/10.1007/s00436-015-4518-8
Pérez Vera C, Kapiainen S, Junnikkala S, Aaltonen K, Spillmann T, Vapalahti O (2014) Survey of selected tick-borne diseases in dogs in Finland. Parasit Vectors 7:285. https://doi.org/10.1186/1756-3305-7-285
Sainz Á, Roura X, Miró G, Estrada-Peña A, Kohn B, Harrus S, Solano Gallego L (2015) Guideline for veterinary practitioners on canine ehrlichiosis and anaplasmosis in Europe. Parasit Vectors 8:75. https://doi.org/10.1186/s13071-015-0649-0
Savić S, Vidić B, Grgić Z, Potkonjak A, Spasojevic L (2014) Emerging vector-borne diseases - incidence through vectors. Front Public Health 2:267. https://doi.org/10.3389/fpubh.2014.00267
Sherifi K, Rexhepi A, Berxholi K, Mehmedi B, Gecaj MR, Hoxha Z, Joachim A, Duscher GG (2018) Crimean–Congo hemorrhagic fever virus and Borrelia burgdorferi sensu lato in ticks from Kosovo and Albania. Front Vet Sci 5:38. https://doi.org/10.3389/fvets.2018.00038
Simón F, Siles-Lucas M, Morchón R, González-Miguel J, Mellado I, Carretón E, Montoya-Alonso JA (2012) Human and animal dirofilariasis: the emergence of a zoonotic mosaic. Clin Microbiol Rev 25:507–544. https://doi.org/10.1128/CMR.00012-12
World Health Organization (2004) Health statistics and information systems. The global burden of disease. https://www.who.int/healthinfo/global_burden_disease/2004_report_update/en/, accessed 11 May 2020
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Sinani, A., Aliu, H., Latifi, F. et al. First serological evidence of infections with selected vector-borne pathogens in dogs in Kosovo. Parasitol Res 119, 3863–3868 (2020). https://doi.org/10.1007/s00436-020-06894-y
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DOI: https://doi.org/10.1007/s00436-020-06894-y