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Colony formation of Blastocystis hominis in soft agar

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Abstract

 This is the first description of a method for growing axenized Blastocystis hominis as colonies in petri dishes containing soft agar. Blastocystis cells cultured in two types of agar appeared to show different colonial morphologies as well as differing colony yields. Microscopic examination of the colonies revealed many amoeboid and giant cells. Many cells were also shown to possess thin filament-like structures that appeared to stretch across the central vacuole.

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Authors and Affiliations

  1. Department of Microbiology, Faculty of Medicine, National University of Singapore, Kent Ridge, Singapore 0511, Republic of Singapore, fax: (65) 77 66 872, , , , , , SG

    S. W. Tan, M. Singh, E. H. Yap, L. C. Ho, K. T. Moe, J. Howe & G. C. Ng

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  1. S. W. Tan
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  2. M. Singh
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  3. E. H. Yap
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  4. L. C. Ho
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  5. K. T. Moe
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  6. J. Howe
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  7. G. C. Ng
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Received: 25 May 1995 / Accepted: 29 August 1995

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Tan, S., Singh, M., Yap, E. et al. Colony formation of Blastocystis hominis in soft agar. Parasitol Res 82, 375–377 (1996). https://doi.org/10.1007/s004360050130

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  • DOI: https://doi.org/10.1007/s004360050130

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