Abstract
A functionalized polystyrene nanofiber (PSNF) immobilized β-galactosidase assembly (PSNF-Gal) was synthesized as a nanobiocatalyst aiming to enhance the biocatalyst stability and functional ability. The PSNF fabricated by electrospinning was functionalized through a chemical oxidation method for enzyme binding. The bioengineering performance of the enzyme carriers was further evaluated for bioconversion of lactose to galacto-oligosaccharides (GOS). The modified PSNF-Gal demonstrated distinguished performances to preserve the same activity as the free β-galactosidase at the optimum pH of 7.0, and to enhance the enzyme stability of PSNF-Gal in an alkaline condition up to pH 10. The PSNF assembly demonstrated improved thermal stability from 37 to 60 °C. The nanobiocatalyst was able to retain 30 % of its initial activity after ninth operation cycles comparing to four cycles with the unmodified counterpart. In contrast with free β-galactosidase, the modified PSNF-Gal enhanced the GOS yield from 14 to 28 %. These findings show the chemically modified PSNF-based nanobiocatalyst may be pertinent for various enzyme-catalysed bioprocessing applications.
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Acknowledgments
MM gratefully acknowledges the financial support from the Universiti Malaysia Sabah and the Malaysian Government. HZ thanks for the support from 111 Project (B12034). BHC acknowledges the support from the State Key Program of National Natural Science Foundation of China (No. 21336009). HPLC facility support from Paul Grbin’s research group and sugar analysis assistance by Nick Van Holst are highly appreciated. MM is also thankful for the technical supports by Jason Peak, Jeffrey Hiorns and Michael Jung from workshop department at School of Chemical Engineering.
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Misson, M., Jin, B., Chen, B. et al. Enhancing enzyme stability and metabolic functional ability of β-galactosidase through functionalized polymer nanofiber immobilization. Bioprocess Biosyst Eng 38, 1915–1923 (2015). https://doi.org/10.1007/s00449-015-1432-5
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DOI: https://doi.org/10.1007/s00449-015-1432-5