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Development of a bioreactor based on magnetically stabilized fluidized bed for bioartificial liver

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Abstract

Bioartificial liver (BAL) based on microcapsules has been proposed as a potential treatment for acute liver failure. The bioreactors used in such BAL are usually expected to achieve sufficient flow rate and minimized void volume for effective application. Due to the superiorities in bed pressure drop and operation velocity, magnetically stabilized fluidized beds (MSFBs) show the potential to serve as ideal microcapsule-based bioreactors. In the present study, we attempted to develop a microcapsule-based MSFB bioreactor for bioartificial liver device. Compared to conventional-fluidized bed bioreactors, the bioreactor presented here increased perfusion velocity and decreased void volume significantly. Meanwhile, the mechanical stability as well as the immunoisolation property of magnetite microcapsules were well maintained during the fluidization. Besides, the magnetite microcapsules were found no toxicity to cell survival. Therefore, our study might provide a novel approach for the design of microcapsule-based bioartificial liver bioreactors.

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Abbreviations

BAL:

Bioartificial liver

MSFB:

Magnetically stabilized fluidized bed

DC:

Direct current

S w :

Swelling degree

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Acknowledgments

This work was supported by the National Natural Science Foundation of China under Grant Numbers 51103157, 81101757, and 81171550, Strategic Priority Research Program of the Chinese Academy of Sciences (No: XDA01030303).

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    Authors and Affiliations

    1. Laboratory of Biomedical Material Engineering, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, 457 Zhongshan Road, Dalian, 116023, China

      Fei Deng, Li Chen, Ying Zhang, Shan Zhao, Yu Wang, Na Li, Shen Li, Xin Guo & Xiaojun Ma

    2. University of Chinese Academy of Sciences, 19 Yuquan Road, Beijing, 100049, China

      Fei Deng, Li Chen, Shan Zhao, Yu Wang, Na Li & Shen Li

    Authors
    1. Fei Deng
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    2. Li Chen
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    3. Ying Zhang
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    5. Yu Wang
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    6. Na Li
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    7. Shen Li
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    8. Xin Guo
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    9. Xiaojun Ma
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    Correspondence to Ying Zhang or Xiaojun Ma.

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    F. Deng and L. Chen are co-first authors and contributed equally.

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    Deng, F., Chen, L., Zhang, Y. et al. Development of a bioreactor based on magnetically stabilized fluidized bed for bioartificial liver. Bioprocess Biosyst Eng 38, 2369–2377 (2015). https://doi.org/10.1007/s00449-015-1472-x

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    • DOI: https://doi.org/10.1007/s00449-015-1472-x

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