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Improving the production yield and productivity of 1,3-dihydroxyacetone from glycerol fermentation using Gluconobacter oxydans NL71 in a compressed oxygen supply-sealed and stirred tank reactor (COS-SSTR)

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Abstract

In this study, a compressed oxygen gas supply was connected to a sealed aerated stirred tank reactor (COS-SSTR) bio-system, leading to a high-oxygen pressure bioreactor used to improve the bio-transformative performance in the production of 1,3-dihydroxyacetone (DHA) from glycerol using Gluconobacter oxydans NL71. A concentration of 301.2 ± 8.2 g L−1 DHA was obtained from glycerol after 32 h of fed-batch fermentation in the COS-SSTR system. The volumetric productivity for this process was 9.41 ± 0.23 g L−1 h−1, which is presently the highest obtained level of glycerol bioconversion into DHA. These results show that the application of this bioreactor would enable microbial production of DHA from glycerol at the industrial scale.

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Acknowledgments

The research was supported by the National Natural Science Funding of China (31370573) and the Key Research and Development Program of Jiangsu (BE2015758). Also, the authors gratefully acknowledge financial support from the Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD) and the Doctorate Fellowship Foundation of Nanjing Forestry University for supporting the work presented in this paper.

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Authors and Affiliations

  1. Jiangsu Co-Innovation Center of Efficient Processing and Utilization of Forest Resources, Nanjing Forestry University, Nanjing, 210037, People’s Republic of China

    Xin Zhou, Xuelian Zhou, Yong Xu & Shiyuan Yu

  2. College of Chemical Engineering, Nanjing Forestry University, Nanjing, 210037, People’s Republic of China

    Xin Zhou, Xuelian Zhou, Yong Xu & Shiyuan Yu

  3. Jiangsu Province Key Laboratory of Green Biomass-based Fuels and Chemicals, Nanjing, 210037, People’s Republic of China

    Xin Zhou, Xuelian Zhou, Yong Xu & Shiyuan Yu

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  1. Xin Zhou
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  2. Xuelian Zhou
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  3. Yong Xu
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  4. Shiyuan Yu
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Correspondence to Yong Xu.

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Zhou, X., Zhou, X., Xu, Y. et al. Improving the production yield and productivity of 1,3-dihydroxyacetone from glycerol fermentation using Gluconobacter oxydans NL71 in a compressed oxygen supply-sealed and stirred tank reactor (COS-SSTR). Bioprocess Biosyst Eng 39, 1315–1318 (2016). https://doi.org/10.1007/s00449-016-1595-8

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  • DOI: https://doi.org/10.1007/s00449-016-1595-8

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