Abstract
In the present work, we have investigated biochemical thermo-kinetic stability of lipases immobilized on a biocompatible polymeric material. Immobilization of lipase Candida rugosa (CRL) was carried out on biocompatible blend of poly vinyl alcohol (PVA) and chitosan (CHY) support via entrapment and glutardehyde (Glu) cross-linking method to produce PVA:CHY:CRL and PVA:CHY:Glu:CRL as robust biocatalyst. These immobilized lipases were characterized by various physico-biochemical characterization techniques. Later on, thermal and solvent stability of polymer immobilized lipase was determined in term of half-life time (t 0.5), D values, enthalpy (ΔH°), entropy (ΔS°), and free energy (ΔG°) of deactivation at different temperatures and in various solvents. The thermodynamic deactivation stability trend was found as: cross-linked lipase CRL > entrapped lipase CRL > free lipase CRL. Moreover, kinetic parameters, such as K m, V max, and catalytic efficiency, were also determined to understand the kinetic features. The polymer immobilized enzyme was reused to investigate the economic viability of the developed biocatalyst.
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Abbreviations
- PVA:
-
Polyvinyl alcohol
- CHY:
-
Chitosan
- PVA:CHY:
-
Control matrix
- PVA:CHY:Glu:
-
Activated glutaraldehyde matrix
- PVA:CHY:CRL:
-
Entrapped lipase
- PVA:CHY:CRL:Glu:
-
Immobilized cross-linked lipase
- t 0.5 :
-
Half life time
- K d :
-
Deactivation rate constant
- D-value:
-
Decimal reduction value
- ΔH°:
-
Enthalpy of deactivation
- ΔG°:
-
Gibb’s free energy of deactivation
- ΔS°:
-
Entropy of deactivation
- Ea:
-
Activation energy
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Author Kirtikumar is grateful to the Council of Scientific & Industrial Research (CSIR), India (File No. 09/991(0015)/2011-EMR-I) for providing research fellowship and fund.
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Badgujar, K.C., Bhanage, B.M. Investigation of deactivation thermodynamics of lipase immobilized on polymeric carrier. Bioprocess Biosyst Eng 40, 741–757 (2017). https://doi.org/10.1007/s00449-017-1740-z
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DOI: https://doi.org/10.1007/s00449-017-1740-z