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Whole-cell bioconversion of naringenin to high added value hydroxylated compounds using Yarrowia lipolytica 2.2ab in surface and liquid cultures

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Abstract

The bioconversion process of bioactive naringenin by whole-cells of Yarrowia lipolytica 2.2ab for the production of increased value-added compounds is successfully achieved in surface and liquid cultures. This approach is an alternative to the commercial production of these bioactive compounds from vegetable sources, which are limited due to their low concentrations and the complexity of the purification processes. The experimentation rendered seven value-added compounds in both surface and liquid bioconversion cultures. Some of the compounds produced have not been previously reported as products from the bioconversion processes, such as the case of ampelopsin. Biosynthetic pathways were suggested for the naringenin bioconversion using whole-cells of Y. lipolytica 2.2ab. Finally, the extracts obtained from the naringenin bioconversion in liquid cultures showed higher percentage of inhibition of DPPH· and ABTS· radicals up to 32.88 and 2.08 times, respectively, compared to commercial naringenin.

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Acknowledgments

We are grateful to the National Council of Science and Technology (Conacyt México) for financial support for project SEP-CONACyT-2013-CO1-220867, and through a Christian Hernández Guzmán PhD Grant 302006.

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Authors and Affiliations

  1. Departamento de Biotecnología, Universidad Autónoma Metropolitana, Iztapalapa, P.A. 55-535, 09340, Mexico City, Mexico

    Christian Hernández-Guzmán, Arely Prado-Barragán, Angélica Román-Guerrero & Sergio Huerta-Ochoa

  2. Facultad de Química, Universidad Nacional Autónoma de México, Mexico City, Mexico

    Miquel Gimeno

  3. Departamento de Química-Bioquímica, Tecnológico Nacional de México, Instituto Tecnológico de Durango, Durango, Mexico

    Olga Miriam Rutiaga-Quiñones & Nuria Elizabeth Rocha Guzmán

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  1. Christian Hernández-Guzmán
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Hernández-Guzmán, C., Prado-Barragán, A., Gimeno, M. et al. Whole-cell bioconversion of naringenin to high added value hydroxylated compounds using Yarrowia lipolytica 2.2ab in surface and liquid cultures. Bioprocess Biosyst Eng 43, 1219–1230 (2020). https://doi.org/10.1007/s00449-020-02316-6

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