Abstract
Background: We devised a standardized animal model to study the impact of laparoscopic colorectal surgery on intrahepatic tumor cell growth.
Methods: The technique of laparoscopic surgery in the rat was extended by endoscopic inoculation of colon cancer cells (CC531) into the portal vein (1 × 104, 5 × 104, 1 × 105, 3 × 105, 5 × 105 cells/ml) of WAG/Rij rats (n= 25). As controls, five animals underwent laparotomy and open intraportal inoculation of 5 × 104 cells/ml.
Results: Hepatic tumor growth occurred after inoculation of 5 × 104, 1 × 105, 3 × 105, and 5 × 105 cells/ml. Extrahepatic tumor and conflating hepatic tumor was observed after the inoculation of 1 × 105, 3 × 105, and 5 × 105 cells/ml. Concentrations of 5 × 104 cells/ml injected either laparoscopically or via an open technique led to single hepatic tumor nodules. No tumor growth was seen after inoculation of 1 × 104 cells/ml.
Conclusions: Laparoscopic intraportal tumor cell inoculation is a feasible technique to create hepatic metastases. The inoculation of 5 × 104 CC531 cells leads to reliable cell growth that can be used to investigate the impact of various laparoscopic techniques on tumor spread.
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Received: 11 June 1998/Accepted: 13 May 1999
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Gutt, C., Kim, Z., Gessmann, T. et al. Hepatic tumor spread of colorectal cancer in a laparoscopic animal model. Surg Endosc 14, 448–451 (2000). https://doi.org/10.1007/s004640000159
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DOI: https://doi.org/10.1007/s004640000159