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GintABC1 encodes a putative ABC transporter of the MRP subfamily induced by Cu, Cd, and oxidative stress in Glomus intraradices

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Abstract

A full-length cDNA sequence putatively encoding an ATP-binding cassette (ABC) transporter (GintABC1) was isolated from the extraradical mycelia of the arbuscular mycorrhizal fungus Glomus intraradices. Bioinformatic analysis of the sequence indicated that GintABC1 encodes a 1513 amino acid polypeptide, containing two six-transmembrane clusters (TMD) intercalated with sequences characteristics of the nucleotide binding domains (NBD) and an extra N-terminus extension (TMD0). GintABC1 presents a predicted TMD0-(TMD-NBD)2 topology, typical of the multidrug resistance-associated protein subfamily of ABC transporters. Gene expression analyses revealed no difference in the expression levels of GintABC1 in the extra- vs the intraradical mycelia. GintABC1 was up-regulated by Cd and Cu, but not by Zn, suggesting that this transporter might be involved in Cu and Cd detoxification. Paraquat, an oxidative agent, also induced the transcription of GintABC1. These data suggest that redox changes may be involved in the transcriptional regulation of GintABC1 by Cd and Cu.

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Acknowledgements

This work was funded by the EU project Genomyca (QLK5-CT-2000-01319) and by a grant from the Consejería de Innovación, Ciencia y Empresa of the Junta de Andalucía, Spain (P06-CVI-02263). Karim Benabdellah was supported by an I3P contract from the Spanish Council for Scientific Research (CSIC).

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  1. Manuel González-Guerrero

    Present address: Department of Chemistry and Biochemistry, Worcester Polytechnic Institute, Worcester, MA, 01609, USA

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  1. Departamento de Microbiología del Suelo y Sistemas Simbióticos, Estación Experimental del Zaidín, Consejo Superior de Investigaciones Científicas, c/Profesor Albareda 1, 18008, Granada, Spain

    Manuel González-Guerrero, Karim Benabdellah, Ascensión Valderas, Concepción Azcón-Aguilar & Nuria Ferrol

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  1. Manuel González-Guerrero
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Correspondence to Nuria Ferrol.

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González-Guerrero, M., Benabdellah, K., Valderas, A. et al. GintABC1 encodes a putative ABC transporter of the MRP subfamily induced by Cu, Cd, and oxidative stress in Glomus intraradices. Mycorrhiza 20, 137–146 (2010). https://doi.org/10.1007/s00572-009-0273-y

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