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Blood metabolite profile in Holstein-Friesian cows fed diets varying in dry matter and metabolizable energy density during early lactation

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Abstract

Dietary effects on circulating concentrations of some blood metabolites were assessed in Holstein-Friesian cows in early lactation. Thirty-two lactating cows were randomly assigned to receive four diets differing in dry matter intake (DMI) and metabolizable energy (ME) density, namely low dry matter and metabolizable energy (LL), low dry matter and high metabolizable energy (LH), high dry matter and low metabolizable energy (HL), high dry matter and high metabolizable energy (HH), and concentrations of some blood metabolites determined. At the end of the 5-week study, the level of insulin-like growth factor-I (IGF-I) was higher (P < 0.001) in cows on diet HH than those on diets HL, LH, or LL. The glucose concentration was higher (P < 0.001) in cows on HH and LH than those on HL and LL diets, while those of non-esterified fatty acid (NEFA) were similar in cows on the four dietary treatments. Cows fed HL and LL diets recorded higher (P < 0.001) beta-hydroxybutyrate (BHB) concentrations than those on the HH or LH diets. Urea concentration was higher (P < 0.001) in cows on LL than that of their counterparts on the other diets. IGF-I was positively correlated (P < 0.01) with glucose, but negatively related with BHB (P < 0.01) and urea (P < 0.05). Glucose was negatively correlated with BHB (P < 0.01) and urea (P < 0.05). NEFA was negatively correlated with urea (P < 0.05) while BHB was positively correlated with urea (P < 0.01). The levels of metabolites in the blood can reflect the nutritional status of cows during early lactation.

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References

  • Anderson JB, Friggens NC, Larsen T, Vestergaard M, Ingvartsen KL (2004) Effect of energy density in the diet and milking frequency on plasma metabolites and hormones in early lactation dairy cows. J Veterinary Med Ser A 51:52–57

    Article  Google Scholar 

  • Butler WR (2005) Inhibition of ovulation in the postpartum cow and the lactating sow. Livest Prod Sci 98:5–12

    Article  Google Scholar 

  • Butler ST (2014) Nutritional management to optimize fertility of dairy cows in pasture-based systems. Animal 8(Supplement 1):15–26

    Article  PubMed  CAS  Google Scholar 

  • Chilliard Y, Bocquier F, Doreau M (1998) Digestive and metabolic adaptations of ruminants to undernutrition, and consequences on reproduction. Reprod Nutr Dev 38:131–152

    Article  PubMed  CAS  Google Scholar 

  • Cieslak A, El-Sherbiny M, Azczechowiakl J, Kowalczyk D, Pers-kamczyc E, Bryszak M, Szulc P, Jóźwik A, Szumacher-Strabel M (2015) Rapeseed and fish oil mixtures supplied at low dose can modulate milk fatty acid composition without affecting rumen fermentation and productive parameters in dairy cows. Anim Sci Paper Rep 33:357–372

    CAS  Google Scholar 

  • Clark CEF, Fulkerson WJ, Nandra KS, Barchia I, Macmillan KL (2005) The use of indicators to assess the degree of mobilization of body reserves in dairy cows in early lactation on a pasture-based diet. Livest Prod Sci 94:199–211

    Article  Google Scholar 

  • Clarke T, Flinn PC, McGowan AA (1982) Low cost pepsin-cellulase assays for the prediction of digestibility of herbage. Grassland Forage Sci 37:147–150

    Article  Google Scholar 

  • Drackley JK, Cardoso FC (2014) Prepartum and postpartum nutritional management to optimize fertility in high-yielding dairy cows in confined TMR systems. Animal 8(Supplement 1):5–14

    Article  PubMed  CAS  Google Scholar 

  • Esposito G, Irons PC, Webb EC (2014) Interactions between negative energy balance, metabolic diseases, uterine health and immune response in transition dairy cows. Anim Reprod Sci 144:60–71

    Article  PubMed  CAS  Google Scholar 

  • Ferguson JD, Galligan DT, Thomas N (1994) Principal descriptors of body condition score in Holstein cows. J Dairy Sci 77:2695–2703

    Article  PubMed  CAS  Google Scholar 

  • Gábor G, Balogh OG, Kern L, Gábor PR, Fébel H (2016) Nutrition, metabolic status and reproductive efficiency in dairy herds. Open J Anim Sci 6(01):75–84

    Article  CAS  Google Scholar 

  • Garro CJ, Mian L, Roldan MC (2014) Subclinical ketosis in dairy cow: prevalence and risk factors in grazing production systems. Anim Physiol Anim Nutr 98:838–844

    Article  CAS  Google Scholar 

  • Giuliodori MJ, Delavaud C, Chilliard Y, Becuvillalobos D, Lacau-mengido I, dela Sota RL (2011) High NEFA concentrations around parturition are associated with delayed ovulations in grazing dairy cows. Livest Sci 141:123–128

    Article  Google Scholar 

  • Grala TM, Lucy MC, Phyn CVC, Sheahan AJ, Mee JM, Roche JR (2011) Somatotropic axis and concentrate supplementation in grazing dairy cows of genetically diverse origin. J Dairy Sci 94:303–315

    Article  PubMed  CAS  Google Scholar 

  • Greenwood PL, Hunt AS, Slepetis RM, Finnerty KD, Alston C, Beermann DH, Bell AW (2002) Effects of birth weight and postnatal nutrition on neonatal sheep III. Regulation of energy metabolism. J Anim Sci 80:2850–2861

    Article  PubMed  CAS  Google Scholar 

  • Jorritsma R, Wensing T, Kruip TA, Vos PL, Noordhuizen JP (2003) Metabolic changes in early lactation and impaired reproductive performance in dairy cows. Vet Res 34:11–26

    Article  PubMed  CAS  Google Scholar 

  • Konigsson K, Savoini G, Govoni N, Invernizzi G, Prandi A, Kindahl H, Veronesi MC (2008) Energy balance, leptin, NEFA and IGF-I plasma concentrations and resumption of postpartum ovarian activity in Swedish red and white breed cows. Acta Vet Scand 50:1–7

    Article  CAS  Google Scholar 

  • Kuhla B, Metges CC, Hammon HM (2016) Endogenous and dietary lipids influencing feed intake and energy metabolism of periparturient dairy cows. Domest Anim Endocrinol 56:S2–S10

    Article  PubMed  CAS  Google Scholar 

  • Lake SL, Scholljegerdes EJ, Hallford DM, Moss GE, Rule DC, Hess BW (2006) Effects of body condition score at parturition and postpartum supplemental fat on metabolite and hormone concentrations of beef cows and their suckling calves. J Anim Sci 84:1038–1047

    Article  PubMed  CAS  Google Scholar 

  • Lee AJ, Twardock AR, Bubar RH, Hall JE, Davis CL (1978) Blood metabolic profiles: their use and relation to nutritional status of dairy cows. J Dairy Sci 61:1652–1670

    Article  PubMed  CAS  Google Scholar 

  • Mashek DG, Ingvartsen KL, Andersen JB, Vestergaard M, Larsen T (2001) Effects of a four-day hyperinsulinemic-euglycemic clamp in early and mid-lactation dairy cows on plasma concentrations of metabolites, hormones, and binding proteins. Domest Anim Endocrinol 21:169–185

    Article  PubMed  CAS  Google Scholar 

  • Mcart JA, Nydam DV, Oetzel GR (2012) Epidemiology of subclinical ketosis in early lactation dairy cattle. J Dairy Sci 95:5056–5066

    Article  PubMed  CAS  Google Scholar 

  • Murphy JJ (1999) The effects of increasing the proportion of molasses in the diet of milking cows on milk production and composition. Anim Feed Sci Technol 79:189–198

    Article  Google Scholar 

  • Ndlovu T, Chimonyo M, Okoh AI, Muchenje V, Dzama K, Raats JG (2007) Assessing the nutritional status of beef cattle: current practices and future prospects. Afr J Biotechnol 6:2727–2734

    Article  CAS  Google Scholar 

  • Obese FY (2003) Concentrations of insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-3 (IGFBP-3) in the plasma and milk of pasture-fed dairy cows in early lactation. PhD thesis, School of Agriculture and Food Systems, University of Melbourne, Australia

  • Obese FY, Humphrys S, Macmillan KL, Egan AR (2008a) Measuring concentrations of insulin-like growth factor-I with an enzyme-linked immunosorbent antibody assay in plasma samples from Holstein cows. J Dairy Sci 91:160–168

    Article  PubMed  CAS  Google Scholar 

  • Obese FY, Rabiee AR, Macmillan KL, Egan AR, Humphrys S, Anderson GA (2008b) Variation in plasma concentrations of insulin-like growth factor-I in pasture-fed Holstein cows. J Dairy Sci 91:1814–1821

    Article  PubMed  CAS  Google Scholar 

  • Pedernera M, Garcia S, Horagadoga A, Barchia I, Fulkerson W (2008) Energy balance and reproduction on dairy cows fed to achieve low or high milk production on a pasture-based system. J Dairy Sci 91:3896–3907

    Article  PubMed  CAS  Google Scholar 

  • SAS (2003) SAS/STAT user guide. SAS. Inst. Inc., Cary

    Google Scholar 

  • Spicer LJ, Tucker WB, Adams GD (1990) Insulin-like growth factor-I in dairy cows. Relationships among energy balance, body condition, ovarian activity, and estrous behaviour. J Dairy Sci 73:929–937

    Article  PubMed  CAS  Google Scholar 

  • SPSS (2007) SPSS for windows version 16.0. Chicago, Illinois, U.S. A

  • Suthar VS, Canelas-Raposo J, Deniz A, Heuwieser W (2013) Prevalence of subclinical ketosis and relationships with postpartum diseases in European dairy cows. J Dairy Sci 96:2925–2938

    Article  PubMed  CAS  Google Scholar 

  • Thissen JP, Ketelslegers JM, Underwood LE (1994) Nutritional regulation of the insulin-like growth factors. Endocr Rev 15:80–101

    PubMed  CAS  Google Scholar 

  • Urdl M, Gruber L, Obritzhauser W, Schauer A (2015) Metabolic parameters and their relationship to energy balance in multiparous Simmental, Brown Swiss and Holstein cows in the periparturient period as influenced by energy supply pre- and post-calving. J Anim Physiol Anim Nutr 99:174–189

    Article  CAS  Google Scholar 

  • Walsh SW, Williams EJ, Evans ACO (2011) A review of the causes of poor fertility in high milk producing dairy cows. Anim Reprod Sci 123:127–138

    Article  PubMed  CAS  Google Scholar 

  • Wathes DC, Chen Z, Bourne N, Taylor VJ, Coffey MP, Brotherstone S (2007) Differences between primiparous and multiparous dairy cows in the inter-relationships between metabolic traits, milk yield and body condition score in the periparturient period. Domest Anim Endocrinol 33:203–225

    Article  PubMed  CAS  Google Scholar 

  • Whitaker DA, Kelly JM, Smith EJ (1983) Subclinical ketosis and serum beta-hydroxybutyrate levels in dairy cattle. Br Vet J 139:462–463

    Article  PubMed  CAS  Google Scholar 

  • Zulu VC, Sawamukai Y, Nakada K, Kida K, Moriyoshi M (2002) Relationships among insulin-like growth factor-I, blood metabolites and post-partum ovarian function in dairy cows. J Vet Med Sci 64:879–885

    Article  PubMed  CAS  Google Scholar 

Download references

Funding

Appreciation is expressed to the University of Melbourne, Dairy Australia, Primegro Ltd. (Adelaide) and the Cooperative Research Centre for Tissue Growth and Repair (Adelaide) for supporting the project.

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Authors and Affiliations

  1. Department of Animal Science, University of Ghana, P.O. Box LG 226, Legon, Ghana

    Frederick Y. Obese

  2. Faculty of Veterinary and Agricultural Sciences, The University of Melbourne, Melbourne, VIC, 3010, Australia

    Frederick Y. Obese, Ahmad Rabiee, Keith Macmillan & Adrian Egan

  3. Primegro Pty Ltd., Thebarton, SA, 5001, Australia

    Simon Humphrys

Authors
  1. Frederick Y. Obese
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  2. Ahmad Rabiee
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  3. Simon Humphrys
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  4. Keith Macmillan
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  5. Adrian Egan
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Correspondence to Frederick Y. Obese.

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All procedures used followed approved guidelines for the ethical treatment of animals.

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The authors declare that they have no conflict of interest.

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Obese, F.Y., Rabiee, A., Humphrys, S. et al. Blood metabolite profile in Holstein-Friesian cows fed diets varying in dry matter and metabolizable energy density during early lactation. Comp Clin Pathol 27, 1191–1197 (2018). https://doi.org/10.1007/s00580-018-2720-7

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  • DOI: https://doi.org/10.1007/s00580-018-2720-7

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