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Flow injection amperometric determination of acetaminophen at a gold nanoparticle modified carbon paste electrode

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Abstract

An amperometric detector with a gold nanoparticle modified carbon paste electrode (GNMCPE) was applied applied to flow injection analysis for the determination of acetaminophen. An obvious shift of the peak potential and increase of the current peak were observed for the GNMCPE in comparison to that of the bare carbon paste electrode. The experimental conditions, such as species of buffer, pH, flow rate, detection volume, injection volume, and injection time were investigated. Under the optimized conditions, the calibration curve was obtained over the concentration range of 0.1–80 mg L−1 of acetaminophen with a linear correlation coefficient of 0.9994. The detection limit (3σ) was estimated to be 0.05 mg L−1 (n = 7). The recoveries of acetaminophen were between 98.40% and 104.1%, and the relative standard deviation varied between 1.66% and 2.74% for the different samples. This method was applied to analyze six types of tablets obtained from a local drugstore. The contents of acetaminophen were found to be 0.498, 0.323, 0.249, 0.324, 0.319 and 0.323 g of each tablet, respectively. These results are consistent with the values obtained by high performance liquid chromatography.

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Acknowledgments

This study was supported by the National Natural Science Foundation of China (20575042, 20775050) and the Science Foundation of the Chinese Education Commission (105141)

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Authors and Affiliations

  1. College of Chemistry, Sichuan University, Chengdu, 610065, People’s Republic of China

    Zemin Xu, Qu Yue & Dan Xiao

  2. College of Chemical Engineering, Sichuan University, Chengdu, 610065, People’s Republic of China

    Zhenjing Zhuang & Dan Xiao

Authors
  1. Zemin Xu
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  2. Qu Yue
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  3. Zhenjing Zhuang
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  4. Dan Xiao
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Corresponding author

Correspondence to Dan Xiao.

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Table S1

Recovery test of acetaminophen sample with amperometric detection on FIA-GNMCPE (n = 5) (DOC 27.5 KB)

Fig. S1

Cyclic voltammograms of the GNMCPE in 50 mg L−1 acetaminophen in potential range between −0.50 and +1.0 V at different scan rate: (a) 0.05, (b) 0.1, (c) 0.2, (d) 0.3, (e) 0.4 V s−1, respectively. Inset: plot of peak current on the square root of the scan rates (DOC 406 KB)

Fig. S2

Observed dependence of current on pH for 0.1 mol L−1 acetaminophen (DOC 39.0 KB)

Fig. S3

Observed dependence of current on phosphate buffer solution concentration (mol L−1) (DOC 42.0 KB)

Fig. S4

Flow injection analysis with amperometric detection results for a GNMCPE in 2.5 mg L−1 acetaminophen, 0.1 mol L−1 phosphate buffer solution (DOC 221 KB)

Fig. S5

Effect of injection volume of sample on a GNMCPE in 2.5 mg L−1 acetaminophen, 0.1 mol L−1 phosphate buffer solution (DOC 42.0 KB)

Fig. S6

Effect of injection time on the magnitude of amperometric response at a GNMCPE in 2.5 mg L−1 acetaminophen, 0.1 mol L−1 phosphate buffer solution (DOC 138 KB)

Fig. S7

Effect of detection volume of flow-through cell for flow injection analysis at a GNMCPE in 2.5 mg L−1 acetaminophen, 0.1 mol L−1 phosphate buffer solution (DOC 74.5 KB)

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Xu, Z., Yue, Q., Zhuang, Z. et al. Flow injection amperometric determination of acetaminophen at a gold nanoparticle modified carbon paste electrode. Microchim Acta 164, 387–393 (2009). https://doi.org/10.1007/s00604-008-0072-8

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  • DOI: https://doi.org/10.1007/s00604-008-0072-8

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