Abstract
We report on a simple, fast and convenient method to engineer lipid vesicles loaded with quantum dots (QDs) by incorporating QDs into a vesicle-type of lipid bilayer using a phase transfer reagent. Hydrophilic CdTe QDs and near-infrared (NIR) QDs of type CdHgTe were incorporated into liposomes by transferring the QDs from an aqueous solution into chloroform by addition of a surfactant. The QD-loaded liposomes display bright fluorescence, and the incorporation of the QDs into the lipid bilayer leads to enhanced storage stability and reduced sensitivity to UV irradiation. The liposomes containing the QD were applied to label living cells and to image mouse tissue in-vivo using a confocal laser scanning microscope, while NIR images of mouse tissue were acquired with an NIR fluorescence imaging system. We also report on the fluorescence resonance energy transfer (FRET) that occurs between the CdTe QDs (the donor) and the CdHgTe QDs (the acceptor), both contained in liposomes. Based on these data, this NIR FRET system shows promise as a tool that may be used to study the release of drug-loaded liposomes and their in vivo distribution.
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Oszwałdowski S, Zawistowska-Gibuła K, Roberts KP (2012) Characterization of CdSe nanocrystals coated with amphiphiles. A capillary electrophoresis study. Microchim Acta 176:345–358
Medintz IL, Mattoussi H, Clapp AR (2008) Potential clinical applications of quantum dots. Int J Nanomedicine 3:151–167
Koneswaran M, Narayanaswamy R (2012) CdS/ZnS core-shell quantum dots capped with mercaptoacetic acid as fluorescent probes for Hg(II) ions. Microchim Acta 178:171–178
Bakalova R, Zhelev Z, Kokuryo D, Spasov L, Aoki I, Saga T (2011) Chemical nature and structure of organic coating of quantum dots is crucial for their application in imaging diagnostics. Int J Nanomedicine 6:1719–1732
Liu W, Howarth M, Greytak AB, Zheng Y, Nocera DG, Ting AY, Bawendi MG (2008) Compact biocompatible quantum dots functionalized for cellular imaging. J Am Chem Soc 130:1274–1284
Gao X, Cui Y, Levenson RM, Chung LW, Nie S (2004) In vivo cancer targeting and imaging with semiconductor quantum dots. Nat Biotechnol 22:969–976
Wang YQ, Ye C, Wu LH, Hu YZ (2010) Synthesis and characterization of self-assembled CdHgTe/gelatin nanospheres as stable near infrared fluorescent probes in vivo. J Pharm Biomed Anal 53:235–242
Gopalakrishnan G, Danelon C, Izewska P, Prummer M, Bolinger PY, Geissbühler I, Demurtas D, Dubochet J, Vogel H (2006) Multifunctional lipid/quantum dot hybrid nanocontainers for controlled targeting of live cells. Angew Chem Int Ed 45:5478–5483
Al-Jamal WT, Al-Jamal KT, Tian B, Lacerda L, Bomans PH, Frederik PM, Kostarelos K (2008) Lipid-quantum dot bilayer vesicles enhance tumor cell uptake and retention in vitro and in vivo. ACS Nano 2:408–418
Al-Jamal WT, Al-Jamal KT, Bomans PH, Frederik PM, Kostarelos K (2008) Functionalized-quantum-dot-liposome hybrids as multimodal nanoparticles for cancer. Small 4:1406–1415
Torchilin VP (2005) Recent advances with liposomes as pharmaceutical carriers. Nat Rev Drug Discov 4:145–160
Düzgünes N, Nir S (1999) Mechanisms and kinetics of liposome-cell interactions. Adv Drug Deliv Rev 40:3–18
Gasset M, Oñaderra M, Thomas PG, Gavilanes JG (1990) Fusion of phospholipid vesicles produced by the anti-tumour protein x-sarcin. Biochem J 265:815–822
De S, Girigoswami A (2004) Fluorescence resonance energy transfer-a spectroscopic probe for organized surfactant media. J Colloid Interface Sci 271:485–495
Oswald B, Lehmann F, Simon L, Terpetschnig E, Wolfbeis OS (2000) Red laser-induced fluorescence energy transfer in an immunosystem. Anal Biochem 280:272–277
Huang X, El-Sayed IH, Qian W, El-Sayed MA (2006) Cancer cell imaging and photothermal therapy in the near-infrared region by using gold nanorods. J Am Chem Soc 128:2115–2120
Klostranec JM, Chan WCW (2006) Quantum dots in biological and biomedical research: recent progress and present challenges. Adv Mater 18:1953–1964
Clapp AR, Medintz IL, Mauro JM, Fisher BR, Bawendi MG, Mattoussi H (2004) Fluorescence resonance energy transfer between quantum dot donors and dye-labeled protein acceptors. J Am Chem Soc 126:301–310
Wang C, Wu QH, Li CR, Wang Z, Ma JJ, Zang XH, Qin NX (2007) Interaction of tetrandrine with human serum albumin: a fluorescence quenching study. Anal Sci 23:429–433
Eaton DF (1988) Reference materials for fluorescence measurement. Pure Appl Chem 60:1107–1114
Clapp AR, Medintz IL, Mattoussi H (2006) Förster resonance energy transfer investigations using quantum-dot fluorophores. ChemPhysChem 7:47–57
Qiu T, Zhao D, Zhou G, Liang Y, He Z, Liu Z, Peng X, Zhou L (2010) A positively charged QDs-based FRET probe for micrococcal nuclease detection. Analyst 135:2394–2399
Kagan CR, Murray CB, Bawendi MG (1996) Long-range resonance transfer of electronic excitations in close-packed CdSe quantum-dot solids. Phys Rev B 54:8633–8643
Acknowledgments
This work was financially supported by the National Natural Science Foundation of China (NO. 30873197), Graduate Innovation Foundation of Simcere Pharmaceutical Group (NO. 02704042), and Graduate Innovation Foundation of Jiangsu Province (NO. CXLX11_0792).
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Ye, C., Wang, Y., Li, C. et al. Preparation of liposomes loaded with quantum dots, fluorescence resonance energy transfer studies, and near-infrared in-vivo imaging of mouse tissue. Microchim Acta 180, 117–125 (2013). https://doi.org/10.1007/s00604-012-0907-1
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DOI: https://doi.org/10.1007/s00604-012-0907-1