Abstract
We show that copper nanoclusters coated with bovine serum albumin are viable probes for fluorometric determination of Cu(II) ion. The effect is attributed to the paramagnetic nature of Cu(II) bound to BSA. The ions Hg(II) and Fe(III) also give a strong effect but can be discerned by addition of EDTA. The probe can be regenerated by adding an excess of EDTA. The method is capable of detecting Cu(II) in the 0.02–34 μM, and the limit of detection is 1nM. The method was applied to the determination of Cu(II) in spiked water samples. It is rapid, simple, and fairly sensitive. The method may also be applied to detect Hg(II) and Fe(III) if Cu(II) is absent.
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Acknowledgments
This work was financially supported by the Scientific Research Foundation of Education Commission of Hubei Province (Q20111010 and T201101), the Natural Science Foundation of Hubei Province (2011CDB059 and 2011CDA111), Research Fund for the Doctoral Program of Higher Education of China (20114208120006), and National Undergraduate Training Programs for Innovation and Entrepreneurship (201210512006 and 201210512020).
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Zhong, Y., Zhu, J., Wang, Q. et al. Copper nanoclusters coated with bovine serum albumin as a regenerable fluorescent probe for copper(II) ion. Microchim Acta 182, 909–915 (2015). https://doi.org/10.1007/s00604-014-1407-2
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DOI: https://doi.org/10.1007/s00604-014-1407-2