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Ultrasensitive amperometric immunosensor for the prostate specific antigen by exploiting a Fenton reaction induced by a metal-organic framework nanocomposite of type Au/Fe-MOF with peroxidase mimicking activity

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Abstract

To increase the sensitivity of electrochemical sensor, Fe-MIL-88B-NH2 (Fe-MOF) with peroxidase-like activity is designed for the construction of immunoprobe. The Fe-MOF was prepared by one-step hydrothermalf method using 2-aminoterephthalic acid and iron(III) chloride. For the immunoprobe, it was fabricated by gold nanocomposite/Fe-MOF (Au/Fe-MOF) for the immobilization of labeling antibody (the antibody was used to conjuncting with label materials). The thin layer of Methylene Blue (MB) covered by reduced graphene oxide-gold nanocomposites (Au-rGO) serves as a substrate to covalently fix coating antibodies. The MB as a redox-active species was modified on the glass carbon electrode that can give a strong amperometric signal at 0.18 V (vs. Ag/AgCl). With the participation of H2O2, Fe-MOF can induce the Fenton reaction which degrades MB covered by Au-rGO on the substrate. The rest of MB on the surface of electrode becomes oxidized thereby generating a current signal. Square wave voltammetry (SWV) was used to quantify PSA. Under optimal conditions, the immunoassay is stable, specific and reproducible. It has a lower detection limit of 0.13 pg mL−1 (S/N = 3) and a wide analytical range that extends from 0.001 to 100 ng mL−1.

A sandwich-type amperometric immunoassay based on Fe-MOF-induced Fenton reaction was designed for sensitive determination of prostate specific antigen.

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Acknowledgements

This research was financed by grants from the National Natural Science Foundation of China (21673143), Natural Science Foundation of Beijing Municipality (2172016), and Capacity Building for Sci-Tech Innovation-Fundamental Scientific Research Funds (19530050179).

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  1. Jiejie Feng and Huiqiang Wang contributed equally to this work.

Authors and Affiliations

  1. Department of Chemistry, Capital Normal University, Beijing, 100048, China

    Jiejie Feng, Huiqiang Wang & Zhanfang Ma

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  1. Jiejie Feng
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  2. Huiqiang Wang
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Correspondence to Zhanfang Ma.

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Feng, J., Wang, H. & Ma, Z. Ultrasensitive amperometric immunosensor for the prostate specific antigen by exploiting a Fenton reaction induced by a metal-organic framework nanocomposite of type Au/Fe-MOF with peroxidase mimicking activity. Microchim Acta 187, 95 (2020). https://doi.org/10.1007/s00604-019-4075-4

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