Abstract
A novel ultrasensitive surface-enhanced Raman spectroscopy (SERS)-based method was developed for the determination of hyaluronidase (HAase), which was based on hyaluronic acid–coated gold nanoparticles (HA-AuNPs) as a substrate, via a facile one-pot method. The detection mechanism is based on HAase which can hydrolyze HA on HA-AuNPs into hyaluronic acid oligomers, causing the originally uniformly dispersed HA-AuNPs to be disintegrated into many smaller HA-AuNPs. These oligomers in turn increase the surface shielding of AuNPs, resulting in high aggregation tendencies. As a result, the original SERS substrate was disassembled, leading to a weakening of the SERS signal at 1173 cm−1. Malachite green was also used as a Raman probe to detect the change of SERS peak intensity and to quantify HAase. Compared with other methods for the determination of HAase, this method is more convenient and efficient; its determination limit was 0.4 mU mL−1. The recoveries of HAase spiked into human urine samples ranged from 97.2 to 103.9%.
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Funding
This work was supported by the Science and Technology Developing Foundation of Jilin Province of China (No. 20200602047ZP) and Industrial Innovation Funds of Jilin Province of China (No. 2018C034-1).
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Wang, W., Li, D., Zhang, Y. et al. One-pot synthesis of hyaluronic acid–coated gold nanoparticles as SERS substrate for the determination of hyaluronidase activity. Microchim Acta 187, 604 (2020). https://doi.org/10.1007/s00604-020-04566-3
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DOI: https://doi.org/10.1007/s00604-020-04566-3