Abstract
Enterovirus 96 (EV-96) is a recently described genotype in the species Human enterovirus C. So far, only partial genome sequences of this enterovirus type have been available. In this study, we report complete genome sequences for two EV-96 strains isolated from healthy children during enterovirus surveillance in Finland. Sequence analysis revealed substantial nucleotide divergence between EV-96 strains and suggested several recombination events between EV-96 and other HEV-C types.
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Acknowledgments
This work was supported by Sigrid Juselius Foundation, Helsinki, Finland. The Regional Reference Laboratory work is supported by WHO. We thank Tytti Vuorinen (Department of Virology, University of Turku) for providing virus isolates, Carita Savolainen-Kopra and Marja-Leena Simonen for helpful discussions and Päivi Klemola for PAA sequence.
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Nucleotide sequence data reported are available in the DDBJ/EMBL/GenBank databases under the accession numbers FJ751914 and FJ751915.
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705_2009_418_MOESM2_ESM.tif
Phylogenetic trees constructed from the partial VP1 sequences (359 nt) of HEV-C strains. The EV-96 strains sequenced in this study are indicated by arrows. MEGA version 4.0 was used for the sequence alignment, and phylogenetic tree construction was done using the neighbour-joining method (1000 bootstrap replicates) and Tamura-Nei model of substitution (TIF 109 kb)
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Smura, T., Blomqvist, S., Hovi, T. et al. The complete genome sequences for a novel enterovirus type, enterovirus 96, reflect multiple recombinations. Arch Virol 154, 1157–1161 (2009). https://doi.org/10.1007/s00705-009-0418-5
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DOI: https://doi.org/10.1007/s00705-009-0418-5