Abstract
CD16 is a major molecule expressed on NK cells. To directly assess the role of natural killer (NK) cells in dengue virus (DENV) infection in vivo, CD16 antibody-treated tamarins were inoculated with a DENV-2 strain. This resulted in the transient depletion of CD16+ NK cells, whereas no significant effects on the overall levels or kinetics of plasma viral loads and antiviral antibodies were observed in the treated monkeys when compared to control monkeys. It remains elusive whether the CD16− NK subpopulation could play an important role in the control of primary DENV infection.
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Acknowledgments
We would like to give special thanks to members of the Corporation for Production and Research of Laboratory Primates for technical assistance. We also would like to give special thanks to Ms. Tomoko Ikoma and Ms. Mizuho Fujita for technical assistance. Moreover, we appreciate Dr. Keith A. Reimann (the NIH Nonhuman Primate Reagent Resource R24 RR016001, NIAID contact HHSN272200900037C) for providing CD16 antibody. This work was supported by grants from the Ministry of Health, Labor and Welfare of Japan (to Hirofumi Akari and Ichiro Kurane). This research was also supported by the Environment Research and Technology Development Fund (D-1007) from the Ministry of the Environment of Japan (to Tomoyuki Yoshida and Hirofumi Akari).
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The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
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T. Yoshida and T. Omatsu contributed equally to this study.
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Yoshida, T., Omatsu, T., Saito, A. et al. CD16+ natural killer cells play a limited role against primary dengue virus infection in tamarins. Arch Virol 157, 363–368 (2012). https://doi.org/10.1007/s00705-011-1178-6
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DOI: https://doi.org/10.1007/s00705-011-1178-6