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Recovery of avirulent, thermostable Newcastle disease virus strain NDV4-C from cloned cDNA and stable expression of an inserted foreign gene

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Abstract

A reverse genetics system for thermostable Newcastle disease virus (NDV) is not currently available. In this study, we developed a reverse genetics system for the avirulent and thermostable NDV4-C strain. Successful recovery of NDV4-C was achieved by using either T7 RNA polymerase or cellular RNA polymerase II to drive transcription of the full-length virus antigenome from cloned cDNA. The recovered viruses rNDV4-C (T7) and rNDV4-C (CMV) showed similar growth properties, thermostability, and virulence as the parental strain NDV4-C. The potential of rNDV4-C (T7) to serve as a viral vector was assessed by generating a recombinant virus, rNDV4-eGFP, which expressed enhanced green fluorescent protein. The rNDV4-eGFP could stably carry and express eGFP for at least fifteen passages. The reverse genetics system for NDV4-C will make it possible to analyze the genetic elements that determine thermostability and the oncolytic properties of NDV.

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Acknowledgments

We would like to thank Dr. Ming Liu and Dr. Henggui Liu for their providing many constructive suggestions. We further thank Dr. Kawaoka for providing plasmid pCAGGS-T7.

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Authors and Affiliations

  1. Division of Avian Infectious Diseases, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, The Chinese Academy of Agricultural Sciences, No. 427 Maduan Street, Nangang District, Harbin, 150001, China

    Xintao Zhang, Huairan Liu, Peixin Liu, Chenchen Zhao & Xiangang Kong

  2. Central Veterinary Institute of Wageningen UR, Lelystad, Netherlands

    Ben P. H. Peeters

Authors
  1. Xintao Zhang
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  2. Huairan Liu
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  3. Peixin Liu
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  4. Ben P. H. Peeters
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  5. Chenchen Zhao
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  6. Xiangang Kong
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Correspondence to Xiangang Kong.

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Supplementary material 1 (PDF 24 kb)

705_2013_1723_MOESM2_ESM.ppt

Supplementary material 2 Fig. S1 Construction, rescue, and confirmation of the stability of rNDV4-eGFP. A. Construction of infectious clone pV3-eGFP: The FseI restriction enzyme site was introduced into the region between the M and F genes of the pV3-V4 infectious clone by site-directed mutagenesis. The eGFP reporter gene was inserted into the FseI site with the NDV gene start and gene end sequences ahead of the start codon of eGFP. The Kozak sequence is marked in italics. (pro, promoter; ter, terminator; Le, leader; Tr, trailer). B. The rNDV4-eGFP virus was recovered from pV3-eGFP and used to infect BSR T7/5 cells (a). To verify the stability of eGFP in the genome of the rNDV4-eGFP virus, this virus was passaged through 9-day-old embryonated SPF eggs 15 times. The 1st, 5th, 10th, and 15th passages of rNDV4-eGFP-infected BSR T7/5 cells (b, c, d, and e, respectively) are shown. Uninfected cells served as the control (f). The arrows indicate autofluorescence. C. Confirmation of the eGFP gene in the genome of rNDV4-eGFP virus using RT-PCR. M, DL15000 DNA ladder marker; lanes 1 and 3, reactions without RT; lane 2, PCR products from the parental virus NDV4-C, with band size 2967 bp; lanes 4–7, PCR products from the 1st, 5th, 10th, and 15th passages of rNDV4-eGFP viruses, with a band size of 3723 bp; lane 8, PCR products from the plasmid pV3-eGFP, with a band size of 3723 bp. (PPT 245 kb)

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Zhang, X., Liu, H., Liu, P. et al. Recovery of avirulent, thermostable Newcastle disease virus strain NDV4-C from cloned cDNA and stable expression of an inserted foreign gene. Arch Virol 158, 2115–2120 (2013). https://doi.org/10.1007/s00705-013-1723-6

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  • DOI: https://doi.org/10.1007/s00705-013-1723-6

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