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Loss in photosynthesis during senescence is accompanied by an increase in the activity of β-galactosidase in leaves of Arabidopsis thaliana: modulation of the enzyme activity by water stress

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Abstract

The precise nature of the developmental modulation of the activity of cell wall hydrolases that breakdown the wall polysaccharides to maintain cellular sugar homeostasis under sugar starvation environment still remains unclear. In this work, the activity of β-galactosidase (EC 3.2.1.23), a cell-wall-bound enzyme known to degrade the wall polysaccharides, has been demonstrated to remarkably enhance during senescence-induced loss in photosynthesis in Arabidopsis thaliana. The enhancement in the enzyme activity reaches a peak at the terminal phase of senescence when the rate of photosynthesis is at its minimum. Although the precise nature of chemistry of the interface between the decline in photosynthesis and enhancement in the activity of the enzyme could not be fully resolved, the enhancement in its activity in dark and its suppression in light or with exogenous sugars may indicate the involvement of loss of photosynthetic production of sugars as a key factor that initiates and stimulates the activity of the enzyme. The hydrolase possibly participates in the catabolic network of cell wall polysaccharides to produce sugars for execution of energy-dependant senescence program in the background of loss of photosynthesis. Drought stress experienced by the senescing leaves accelerates the decline in photosynthesis with further stimulation in the activity of the enzyme. The stress recovery of photosynthesis and suppression of the enzyme activity on withdrawal of stress support the proposition of photosynthetic modulation of the cell-wall-bound enzyme activity.

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Acknowledgements

The authors wish to thank the Council of Scientific and Industrial Research (CSIR), New Delhi, India, for financial support by a grant to BB under CSIR Emeritus Scientist Project (No. 21 (0886)/12-EMR II).

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  1. Laboratory of Biochemistry and Molecular Biology, School of Life Sciences, Sambalpur University, Jyoti Vihar, Burla, Odisha, 768019, India

    Jitendra Kumar Pandey, Sidhartha Kumar Dash & Basanti Biswal

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  1. Jitendra Kumar Pandey
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  2. Sidhartha Kumar Dash
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  3. Basanti Biswal
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Correspondence to Basanti Biswal.

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Pandey, J.K., Dash, S.K. & Biswal, B. Loss in photosynthesis during senescence is accompanied by an increase in the activity of β-galactosidase in leaves of Arabidopsis thaliana: modulation of the enzyme activity by water stress. Protoplasma 254, 1651–1659 (2017). https://doi.org/10.1007/s00709-016-1061-0

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