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Osteocalcin, but not deoxypyridinoline, increases in response to isoflurane-induced anaesthesia in young female guinea pigs

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Abstract

The effect of the inhaled anaesthetic isoflurane was investigated on bone biomarkers, both during maturation and on minerals and glucose postpartum. Female guinea pigs (n = 10) were anaesthetized during maturation (5 and 9 weeks) and postpartum (26 weeks of age) with isoflurane during dual-energy X-ray absorptiometry scanning. Blood collection was performed at all ages before and after anaesthesia for measurement of plasma osteocalcin (OC), total deoxypyridinoline (tDPD), and cortisol. Postpartum measurements also included: blood ions, acid–base parameters and glucose, plasma minerals, total alkaline phosphatase (tALP), and albumin. Plasma OC concentration almost doubled after exposure to isoflurane at 5 weeks (30.1 ± 5.0–57.9 ± 11.2 nmol/L, p < 0.001) and at 9 weeks (29.1 ± 7.5–62.9 ± 15.9 nmol/L, p < 0.001), but did not change postpartum (3.7 ± 3.3–4.3 ± 3.9 nmol/L, p = 0.88). There was no effect of isoflurane exposure on plasma tDPD at any age. Plasma cortisol increased after exposure to isoflurane at 9 weeks (1859.6 ± 383.2–2748.0 ± 235.3 nmol/L, p < 0.01) and postpartum (3376.7 ± 322.2–4091.6 ± 195.6 nmol/L, p < 0.001) but not at 5 weeks (2088.3 ± 326.4–2464.1 ± 538.0 nmol/L, p > 0.05). Blood ionized Ca2+, Na+ and plasma total Ca did not change, whereas plasma albumin decreased, and inorganic phosphate (PO4) and Cl− increased upon exposure to isoflurane. Isoflurane decreased tALP (43.2 ± 6.6–40.2 ± 5.9 IU/L, p = 0.01) and increased glucose (7.5 ± 0.6–10.9 ± 1.7 mmol/L, p < 0.0001) postpartum. Isoflurane inflates the assessment of a bone-derived biomarker, OC, during rapid growth, but not following pregnancy when formation is very low. Measurements prior to anaesthesia are recommended to reflect normal metabolism.

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Acknowledgments

NT contributed in the design of this study by performing biochemical assessments, interpretation of data, statistical analyses, and article writing. CR and RK performed critical reviews of the manuscript and provided helpful comments on the interpretation of results. RK is in receipt of the Canadian Institutes of Health Research MT10839. HW contributed to the original thought and design of this study and undertook supervision of the biomarker measures and critical review of the manuscript. She is in receipt of a CRC (Canada Research Chair) tier II in Nutrition, Development and Aging, and acknowledges CFI (Canadian Foundation for Innovation) infrastructure support for the DXA. Dr. Weiler wrote the grant along with CR, RK, and NT, which was approved and funded by CIHR (Canadian Institutes of Health Research). Acknowledgements are also given to Sherry Agellon for biochemical assessment of cortisol levels and Nandita Perumal for her assistance during this animal study at the School of Dietetic and Human Nutrition at McGill University.

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All authors have no conflicts of interest.

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Authors and Affiliations

  1. School of Dietetics and Human Nutrition, Macdonald-Stewart Building Macdonald Campus, 21111 Lakeshore Road, Ste-Anne-de-Bellevue, Quebec, H9X 3V9, Canada

    Negar Tabatabaei & Hope A. Weiler

  2. Department of Pediatrics, Montreal Children’s Hospital, McGill University Health Centre, 2300 Tupper St., Montreal, QC, H3H 1P3, Canada

    Celia J. Rodd

  3. Department of Medicine, Royal Victoria Hospital, McGill University Health Center, 687 Pine Avenue West, Montreal, QC, H3A 1A1, Canada

    Richard Kremer

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  1. Negar Tabatabaei
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  2. Celia J. Rodd
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  4. Hope A. Weiler
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Correspondence to Hope A. Weiler.

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Tabatabaei, N., Rodd, C.J., Kremer, R. et al. Osteocalcin, but not deoxypyridinoline, increases in response to isoflurane-induced anaesthesia in young female guinea pigs. J Bone Miner Metab 33, 253–260 (2015). https://doi.org/10.1007/s00774-014-0593-7

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