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Biomonitoring of oral epithelial cells in smokers and non-smokers submitted to panoramic X-ray: comparison between buccal mucosa and lateral border of the tongue

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Abstract

The aim of the present study was to comparatively evaluate DNA damage (micronucleus) and cellular death (pyknosis, karyolysis, and karyorrhexis) in exfoliated oral mucosa cells from smokers and non-smokers submitted to dental X-ray using two anatomic sites: buccal mucosa and lateral border of the tongue. A total of 15 heavy smokers and 17 non-smokers were submitted to panoramic dental radiography for orthodontic reasons. Individuals had epithelial cells from cheek and lateral border of the tongue mechanically exfoliated, placed in fixative, and dropped in clean slides which were checked for the above nuclear phenotypes. The results pointed out no significant statistically differences (p > 0.05) of micronucleated oral mucosa cells before versus after X-ray exposure for both oral sites evaluated either to smokers or to non-smokers. X-ray exposure was able to increase other nuclear alterations closely related to cytotoxicity such as karrhyorexis, pyknosis, and karyolysis for two groups evaluated. Nevertheless, the most pronunciated effects were found to lateral border of the tongue of smokers. In summary, these data indicate that panoramic X-ray is able to induce cellular death in oral mucosa cells. It seems that lateral border of the tongue is more sensitive site to cytotoxic insult induced by ionizing radiation combined with continuous cigarette smoke exposure.

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References

  1. Boeddinghaus R, Whyte A (2008) Current concepts in maxillofacial imaging. Eur J Radiol 66:396–418

    Article  PubMed  Google Scholar 

  2. Lee CK, Munoz JA, Fulp C, Chang KM, Rogers JC, Borgerding MF, Doolittle DJ (1994) Inhibitory activity of cigarette-smoke condensate on the mutagenicity of heterocyclic amines. Mutat Res 322:21–32

    Article  PubMed  Google Scholar 

  3. Gandini S, Botteri E, Iodice S, Boniol M, Lowenfels AB, Maisonneuve P, Boyle P (2008) Tobacco smoking and cancer: a meta-analysis. Int J Cancer 122:155–64

    Article  PubMed  Google Scholar 

  4. Ribeiro DA, Fávero Salvadori DM, da Silva RN, Ribeiro Darros B, Alencar Sarto F, Finotto S, Giacomelli L, Mazzotti D, Tomanin R, Levis AG (1987) The micronucleus assay in exfoliated cells of the human buccal. Mutagenesis 2:11–17

    Article  Google Scholar 

  5. Induslki JA, Lutz W (1997) Molecular epidemiology: cancer risk assessment using biomarkers for detecting early health effects in individuals exposed to occupational and environmental carcinogens. Reviews Environ Health 12:179–190

    Google Scholar 

  6. Sram RJ, Rossner P, Peltonen K, Podrazilova K, Mrackova G, Demopoulos NA, Stephanou D, Vlachodimitropoulos D, Darroudi AD, Tates D (1988) Chromosomal aberrations, sister-chromatid exchanges, cell with high frequency of SCE, micronuclei and comet assay parameteres in 1, 3-butadiene-exposed workers. Mutat Res 419:145–154

    Google Scholar 

  7. Stich HF, Parida BB, Brunnemann KD (1992) Localized formation of micronuclei in the oral mucosa and tobacco-specific nitrosamines in the saliva of "reverse" smokers, Khaini-tobacco chewers and gudakhu users. Int J Cancer 21:172–176

    Article  Google Scholar 

  8. Belien JA, Copper MP, Braakhuis BJ, Snow GB, Baak JP (1995) Standardization of counting micronuclei: definition of a protocol to measure genotoxic damage in human exfoliated cells. Carcinogenesis 16:2395–2400

    Article  PubMed  Google Scholar 

  9. Ribeiro DA, de Oliveira G, de Castro G, Angelieri F (2008) Cytogenetic biomonitoring in patients exposed to dental X-rays: comparison between adults and children. Dentomaxillofac Radiol 37:404–407

    Article  PubMed  Google Scholar 

  10. Sarto F, Finotto S, Giacomelli L, Mazzotti D, Tomanin R, Levis AG (1987) The micronucleus assay in exfoliated cells of the human buccal. Mutagenesis 2:11–17

    Article  PubMed  Google Scholar 

  11. Angelieri A, Oliveira GR, Sannomiya EK, Ribeiro DA (2007) DNA damage and cellular death in oral mucosa cells of children who have undergone panoramic dental radiography. Ped Radiol 37:561–565

    Article  Google Scholar 

  12. Thomas P, Harvey S, Gruner T, Fenech M (2008) The buccal cytome and micronucleus frequency is substantially altered in Down’s syndrome and normal ageing compared to young healthy controls. Mutat Res 638:37–47

    PubMed  Google Scholar 

  13. Fenech M, Holland N, Chang WP, Zeiger E, Bonassi S (1999) The Human MicroNucleus Project—an international collaborative study on the use of the micronucleus technique for measuring DNA damage in humans. Mutat Res 428:271–283

    PubMed  Google Scholar 

  14. Pastor S, Gutierrez S, Creus A, Cebulska-Wasilewska A, Marcos R (2001) Micronuclei in peripheral blood lymphocytes and buccal epithelial cells of Polish farmers exposed to pesticides. Mutat Res 495:147–156

    PubMed  Google Scholar 

  15. Neri M, Fucic A, Knudsen LE, Lando C, Merlo F, Bonassi S (2003) Micronuclei frequency in children exposed to environmental mutagens: a review. Mutat Res 544:243–254

    Article  PubMed  Google Scholar 

  16. He JL, Chen LF, Jin LF, Jin HY (2000) Comparative evaluation of the in vitro micronucleus test and the comet assay for the detection of genotoxic effects of X-ray radiation. Mutat Res 469:223–231

    PubMed  Google Scholar 

  17. Visvardis EE, Tassiou AM, Piperakis SM (1997) Study of DNA damage induction and repair capacity of fresh and cryopreserved lymphocytes exposed to H2O2 and gamma-irradiation with the alkaline comet assay. Mutat Res 383:71–80

    PubMed  Google Scholar 

  18. Holland N, Bolognesi C, Kirsch-Volders M, Bonassi S, Zeiger E, Knasmueller S, Fenech M (2008) The micronucleus assay in human buccal cells as a tool for biomonitoring DNA damage: the HUMN project perspective on current status and knowledge gaps. Mutat Res 659:93–108

    Article  PubMed  Google Scholar 

  19. Speit G, Schmid O (2006) Local genotoxic effects of formaldehyde in humans measured by the micronucleus test with exfoliated epithelial cells. Mutat Res 613:1–9

    Article  PubMed  Google Scholar 

  20. Ribeiro DA, Angelieri F (2008) Cytogenetic biomonitoring of oral mucosa cells from adults exposed to dental X-rays. Radiat Med 26:325–330

    Article  PubMed  Google Scholar 

  21. Nersesyan A, Kundi M, Atefie K, Schulte-Hermann R, Knasmüller S (2006) Effect of staining procedures on the results of micronucleus assays with exfoliated oral mucosa cells. Cancer Epidemiol Biomarkers Prev 15:1835–1840

    Article  PubMed  Google Scholar 

  22. Sugano N, Minigeshi T, Kawamoto K, Ito K (2001) Nicotine inhibits UV-induced activation of the apoptotic pathway. Toxicol Lett 125:61–65

    Article  PubMed  Google Scholar 

  23. Onoda N, Nehemi A, Weiner D, Mujumdar D, Christen R, Los G (2001) Nicotine affects the signaling of the death pathway, reducing the response of head and neck cancer cell lines to DNA damaging agents. Head Neck 23:860–870

    Article  PubMed  Google Scholar 

  24. Schwartz JL, Muscat JE, Baker V, Larlos E, Stephenson GD, Guo W, Xie T, Gu X, Cung FL (2003) Oral cytology assessment by flow cytometry of DNA adducts, aneuploidy, proliferation and apoptosis show differences between smokers and non-smokers. Oral Oncol 39:842–854

    Article  PubMed  Google Scholar 

  25. Argentin G, Chichetti R (2004) Genotoxic and antiapoptotic effect of nicotine on human gingival fibroblasts. Toxicol Sci 79:75–81

    Article  PubMed  Google Scholar 

  26. Cerqueira EM, Gomes-Filho IS, Trindade S, Lopes MA, Passos JS, Machado-Santelli GM (2004) Genetic damage in exfoliated cells from oral mucosa of individuals exposed to X-rays during panoramic dental radiographies. Mutat Res 562:111–117

    PubMed  Google Scholar 

  27. Popova L, Kishkilova D, Hadjidekova V, Hristova R, Atanasova P, Hadjidekova V, Ziya D, Hadjidekov V (2007) Micronucleus test in buccal epithelium cells from patients subjected to panoramic radiography. Dentomaxillofac Radiol 36:168–171

    Article  PubMed  Google Scholar 

  28. Kujan O, Oliver RJ, Khattab A, Roberts SA, Thakker N, Sloan P (2006) Evaluation of a new binary system of grading oral epithelial dysplasia for prediction of malignant transformation. Oral Oncol 42:987–993

    Article  PubMed  Google Scholar 

  29. Mally A, Jagetia JK (2002) Non-genotoxic carcinogens: early effects on gap junctions, cell proliferation and apoptosis in the rat. Toxicology 180:233–248

    Article  PubMed  Google Scholar 

  30. Xu GL, Bestor HH, Bourc’his D, Hsich CL, Tommerup N, Bugge M, Hulten M, Qu X, Russo JJ, Viegas-Pequignot E (1999) Chromosome instability and immunodeficiency syndrome caused by mutations in a DNA methyltransferase gene. Nature 402:187–191

    Article  PubMed  Google Scholar 

  31. Torres-Bugárin O, Ventura-Aguilar A, Zamora-Pilar A, Gomez-Meda BC, Ramos-Ibarra ML, Morgan-Villela G, Gutierrez-Franco G, Zuniga-Gonzalez G (2003) Evaluation of cisplatin  + 5-FU, carboplatin  + 5-FU, and ifosfamide + epirubicine regimens using the micronuclei test and nuclear abnormalities in buccal mucosa. Mutat Res 539:177–186

    PubMed  Google Scholar 

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Acknowledgements

This study was supported by grants from FAPESP (Fundação de Amparo à Pesquisa do Estado de Sao Paulo, grant number: 2007/01228-4). TCGM and DAR are recipients of the CNPq fellowship.

Conflict of interest

The authors declare that they have no conflict of interest.

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Authors and Affiliations

  1. Department of Orthodontics, Sao Paulo Metodista University, UMESP, Sao Bernardo do Campo, SP, Brazil

    Fernanda Angelieri & Tatiana de Cássia Gonçalves Moleirinho

  2. Department of Pathology, Paulista Medical School, Federal University of São Paulo, UNIFESP, Sao Paulo, SP, Brazil

    Viviane Carlin, Celina Tizuko Fujiyama Oshima & Daniel Araki Ribeiro

  3. Department of Biosciences, Federal University of Sao Paulo, UNIFESP, Santos, SP, Brazil

    Daniel Araki Ribeiro

  4. Departamento de Biociências, Universidade Federal de São Paulo, UNIFESP, Av. Ana Costa 95, 11060-001, Santos, SP, Brazil

    Daniel Araki Ribeiro

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  1. Fernanda Angelieri
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  2. Tatiana de Cássia Gonçalves Moleirinho
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  3. Viviane Carlin
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  4. Celina Tizuko Fujiyama Oshima
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Correspondence to Daniel Araki Ribeiro.

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Angelieri, F., de Cássia Gonçalves Moleirinho, T., Carlin, V. et al. Biomonitoring of oral epithelial cells in smokers and non-smokers submitted to panoramic X-ray: comparison between buccal mucosa and lateral border of the tongue. Clin Oral Invest 14, 669–674 (2010). https://doi.org/10.1007/s00784-009-0345-6

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  • DOI: https://doi.org/10.1007/s00784-009-0345-6

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