Abstract
A novel glucose biosensor was fabricated by immobilizing glucose oxidase (GOx) on Ag nanoparticles-decorated multiwalled carbon nanotube (AgNP-MWNT) modified glass carbon electrode (GCE). The AgNP-MWNT composite membrane showed an improving biocompatibility for GOx immobilization and an enhancing electrocatalytic activity toward reduction of oxygen due to decoration of AgNPs on MWNT surfaces. The AgNPs also accelerated the direct electron transfer between redox-active site of GOx and GCE surface because of their excellent conductivity and large capacity for protein loading, leading to direct electrochemistry of GOx. The glucose biosensor of this work showed a lower limit of detection of 0.01 mM (S/N = 3) and a wide linear range from 0.025 to 1.0 mM, indicating an excellent analytical performance of the obtained biosensor to glucose detection. The resulting biosensor exhibits good stability and excellent reproducibility. Such bionanocomposite provides us good candidate material for fabrication of biosensors based on direct electrochemistry of immobilized enzymes.
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References
Wang SG, Zhang Q, Wang RL, Yoon SF (2003) A novel multi-walled carbon nanotube-based biosensor for glucose detection. Biochem Biophys Res Commun 311:572–576
Valcárcel M, Cárdenas S, Simonet BM (2007) Role of carbon nanotubes in analytical science. Anal Chem 79:4788–4797
Wang J, Dai JH, Yarlagadda T (2005) Carbon nanotube-conducting-polymer composite nanowires. Langmuir 21:9–12
Wang T, Hu XG, Dong SJ (2007) Construction of metal nanoparticle/multiwalled carbon nanotube hybrid nanostructures providing the most accessible reaction sites. J Mater Chem 17:4189–4195
Zou YJ, Xiang CL, Sun LX, Xu F (2008) Glucose biosensor based on electrodeposition of platinum nanoparticles onto carbon nanotubes and immobilizing enzyme with chitosan-SiO2 sol-gel. Biosens Bioelectron 23:1010–1016
Zhang MG, Gorski W (2005) Electrochemical sensing platform based on the carbon nanotubes/redox mediators-biopolymer system. J Am Chem Soc 127:2058–2059
Chu X, Duan DX, Shen GL, Yu RQ (2007) Amperometric glucose biosensor based on electrodeposition of platinum nanoparticles onto covalently immobilized carbon nanotube electrode. Talanta 71:2040–2047
Yang MH, Yang YH, Liu YL, Shen GL, Yu RQ (2006) Platinum nanoparticles-doped sol–gel/carbon nanotubes composite electrochemical sensors and biosensors. Biosens Bioelectron 21:1125–1131
Jia JB, Wang BQ, Wu AG, Cheng GJ, Li Z, Dong SJ (2002) A method to construct a third-generation horseradish peroxidase biosensor: self-assembling gold nanoparticles to three-dimensional sol-gel network. Anal Chem 74:2217–2223
Patolsky F, Gabriel T, Willner I (1999) Controlled electrocatalysis by microperoxidase-11 and Au-nanoparticle superstructures on conductive supports. J Electroanal Chem 479:69–73
Li FH, Wang ZH, Shan CS, Song JF, Han DX, Niu L (2009) Preparation of gold nanoparticles/functionalized multiwalled carbon nanotube nanocomposites and its glucose biosensing application. Biosens Bioelectron 24:1765–1770
Xu H, Zeng LP, Xing SJ, Shi GY, Xian YZ, Jin L (2008) Microwave-radiated synthesis of gold nanoparticles/carbon nanotubes composites and its application to voltammetric detection of trace mercury (II). Electrochem Commun 10:1839–1843
Zhang YZ, Zhang KY, Ma HY (2009) Electrochemical DNA biosensor based on silver nanoparticles/poly(3-(3-pyridyl)acrylic acid)/carbon nanotubes modified electrode. Anal Biochem 387:13–19
Yang ZY, Li JP, Fang C (2005) A biosensor based on the glucose oxidase film modified by titanic sol–gel doping with Prussian Blue nanoparticles. Chin J Anal Chem 33:538–542
Yang YH, Yang GM, Huang Y, Bai HP, Lu XX (2009) A new hydrogen peroxide biosensor based on gold nanoelectrode ensembles/multiwalled carbon nanotubes/chitosan film-modified electrode. Colloids Surf A Physicochem Eng Asp 340:50–55
Tsai MC, Tsai YC (2009) Adsorption of glucose oxidase at platinum-multiwalled carbon nanotube-alumina-coated silica nanocomposite for amperometric glucose biosensor. Sensors Actuators B 141:592–598
Zhang J, Gao L (2010) Synthesis of highly dispersed platinum nanoparticles on multiwalled carbon nanotubes and their electrocatalytic activity toward hydrogen peroxide. J Alloy Comp 505:604–608
Qin X, Wang HC, Wang XX, Miao ZY, Chen LL, Zhao W et al (2010) Amperometric biosensors based on gold nanoparticles-decorated multiwalled carbon nanotubes-poly(diallyldimethylammonium chloride) biocomposite for the determination of choline. Sensors Actuators B 147:593–598
Zhang YW, Chang GH, Liu S, Lu WB, Tian JQ, Sun XP (2011) A new preparation of Au nanoplates and their application for glucose sensing. Biosens Bioelectron 28:344–348
Lin JH, He CY, Zhao Y, Zhang SS (2009) One-step synthesis of silver nanoparticles/carbon nanotubes/chitosan film and its application in glucose biosensor. Sensors Actuators B 137:768–773
Qin XY, Lu WB, Luo YL, Chang GH, Asiri AM et al (2012) Synthesis of Ag nanoparticle-decorated 2,4,6-tris(2-pyridyl)-1,3,5-triazine nanobelts and their application for H2O2 and glucose detection. Analyst 137:939–943
Zhang YW, Liu S, Wang L, Qin XY, Tian JQ, Lu WB et al (2012) One-pot green synthesis of Ag nanoparticles-graphene nanocomposites and their applications in SERS, H2O2, and glucose sensing. RSC Adv 2:538–545
Lu WB, Luo YL, Chang GH, Sun XP (2011) Synthesis of functional SiO2-coated graphene oxide nanosheets decorated with Ag nanoparticles for H2O2 and glucose detection. Biosens Bioelectron 26:4791–4797
Gutés A, Carraro C, Maboudian R (2012) Single-layer CVD-grown graphene decorated with metal nanoparticles as a promising biosensing platform. Biosens Bioelectron 33:56–59
Baby TT, Jyothirmayee Aravind SS, Arochiadoss T, Rakhi RB, Ramaprabhu S (2010) Metal decorated graphene nanosheets as immobilization matrix for amperometric glucose biosensor. Sensors Actuators B 145:71–77
Wu H, Wang J, Kang XH, Wang CM, Wang DH, Liu J, Aksay IA, Lin YH (2009) Glucose biosensor based on immobilization of glucose oxidase in platinum nanoparticles/graphene/chitosan nanocomposite film. Talanta 80:403–406
Deng SY, Jian GQ, Lei JP, Hu Z, Ju HX (2009) A glucose biosensor based on direct electrochemistry of glucose oxidase immobilized on nitrogen-doped carbon nanotubes. Biosens Bioelectron 25:373–377
Liu SQ, Ju HX (2003) Reagentless glucose biosensor based on direct electron transfer of glucose oxidase immobilized on colloidal gold modified carbon paste electrode. Biosens Bioelectron 19:177–183
Liu S, Tian JQ, Wang L, Luo YL, Lu WB, Sun XP (2011) Self-assembled graphene platelet-glucose oxidase nanostructures for glucose biosensing. Biosens Bioelectron 26:4491–4496
Cai CX, Chen J (2004) Direct electron transfer of glucose oxidase promoted by carbon nanotubes. Anal Biochem 332:75–83
Salimi A, Compton RG, Hallaj R (2004) Glucose biosensor prepared by glucose oxidase encapsulated sol-gel and carbon-nanotube-modified basal plane pyrolytic graphite electrode. Anal Biochem 333:49–56
Liu X, Shi L, Niu W, Li H, Xu G (2008) Amperometric glucose biosensor based on single-walled carbon nanohorns. Biosens Bioelectron 23:1887–1890
Zhou M, Shang L, Li B, Huang L, Dong S (2008) Highly ordered mesoporous carbons as electrode material for the construction of electrochemical dehydrogenase- and oxidase-based biosensors. Biosens Bioelectron 24:442–447
Chen SH, Yuan R, Chai YQ, Zhang LY, Wang N, Li XL (2007) Amperometric thirdgeneration hydrogen peroxide biosensor based on the immobilization of hemoglobin on multiwall carbon nanotubes and gold colloidal nanoparticles. Biosens Bioelectron 22:1268–1274
Yang J, Yang T, Feng YY, Jiao K (2007) A DNA electrochemical sensor based on nanogold-modified poly-2,6-pyridinedicarboxylic acid film and detection of PAT gene fragment. Anal Biochem 365:24–30
Peng H, Soeller C, Cannell MB, Bowmaker GA, Cooney RP, Sejdic JT (2006) Electrochemical detection of DNA hybridization amplified by nanoparticles. Biosens Bioelectron 21:1727–1736
Reisberg S, Dang LA, Nguyen QA, Piro B, Noel V, Nielsen PE et al (2008) Label-free DNA electrochemical sensor based on a PNA-functionalized conductive polymer. Talanta 76:206–210
Acknowledgments
This work was supported by the National Science Foundation of China (50876058), the Program for Professor of Special Appointment (Eastern Scholar) at Shanghai Institutions of Higher Learning, and the Program for New Century Excellent Talents in University (NCET-10-883).
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Chen, L., Xie, H. & Li, J. Electrochemical glucose biosensor based on silver nanoparticles/multiwalled carbon nanotubes modified electrode. J Solid State Electrochem 16, 3323–3329 (2012). https://doi.org/10.1007/s10008-012-1773-9
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DOI: https://doi.org/10.1007/s10008-012-1773-9