Abstract
I propose a concept of a novel Fourier-transform phase-modulation fluorometer by which a fluorescence decay waveform can be obtained. In the fluorometer, the modulation frequency of the excitation light source is swept continuously from a start frequency fmin to an end frequency fmax with a time duration T. The resultant fluorescence signal waveform is Fourier-transformed to obtain amplitude and phase spectra. The ratio of the amplitude spectrum and the difference of the phase spectrum over those of the reference spectra that are obtained from a non-fluorescent material are calculated, respectively, and the pair of both spectral data is inverse-Fourier-transformed again to obtain the fluorescence decay waveform. To verify and demonstrate the effectiveness of the concept, I carried out (1) numerical simulations, (2) determination of a time constant of a passive resistor-capacitor (RC) differential circuit, and (3) measurement of a fluorescent decay waveform of YAG materials packed in Nichia’s white LED.
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Iwata, T. Proposal for Fourier-Transform Phase-Modulation Fluorometer. OPT REV 10, 31–37 (2003). https://doi.org/10.1007/s10043-003-0031-x
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DOI: https://doi.org/10.1007/s10043-003-0031-x