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Requirement of vesicle-associated membrane protein 721 and 722 for sustained growth during immune responses in Arabidopsis

  • Research Article
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Molecules and Cells

Abstract

Extracellular immune responses to ascomycete and oomycete pathogens in Arabidopsis are dependent on vesicle-associated secretion mediated by the SNARE proteins PEN1 syntaxin, SNAP33 and endomembrane-resident VAMP721/722. Continuous movement of functional GFP-VAMP722 to and from the plasma membrane in non-stimulated cells reflects the second proposed function of VAMP721/722 in constitutive secretion during plant growth and development. Application of the bacterium-derived elicitor flg22 stabilizes VAMP721/722 that are otherwise constitutively degraded via the 26S proteasome pathway. Depletion of VAMP721/722 levels by reducing VAMP721/722 gene dosage enhances flg22-induced seedling growth inhibition in spite of elevated VAMP721/722 abundance. We therefore propose that plants prioritize the deployment of the corresponding secretory pathway for defense over plant growth. Interstingly, VAMP721/722 specifically interact in vitro and in vivo with the plasma membrane syntaxin SYP132 that is required for plant growth and resistance to bacteria. This suggests that the plant growth/immunity-involved VAMP721/722 form SNARE complexes with multiple plasma membrane syntaxins to discharge cue-dependent cargo molecules.

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Author notes

  1. Mark Kwaaitaal

    Present address: Department of Agriculture and Ecology Faculty of Life Sciences, University of Copenhagen, Copenhagen, Denmark

Authors and Affiliations

  1. Deparment of Plant Microbe Interactions, Max-Planck-Institut für Züchtungsforschung, D-50829, Köln, Germany

    Hye Sup Yun, Mark Kwaaitaal, Paul Schulze-Lefert & Chian Kwon

  2. Biological Sciences Major, College of Biological Science and Biotechnology, Konkuk University, Seoul, 143-701, Korea

    Hye Sup Yun

  3. Department of Biological Sciences, Louisiana State University, New Orleans, LA, 70803-1715, USA

    Naohiro Kato

  4. Department of Molecular Biology, Dankook University, Yongin, 448-701, Korea

    Changhyun Yi, Sohyeon Park & Chian Kwon

  5. Laboratory of Cellular Dynamics, Graduate School of Life and Environmental Sciences, Kyoto Prefectural University, Kyoto, 606-8522, Japan

    Masa H. Sato

  6. Department of Integrated Molecular Sciences, Dankook University, Yongin, 448-701, Korea

    Chian Kwon

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  1. Hye Sup Yun
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Correspondence to Paul Schulze-Lefert or Chian Kwon.

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Yun, H.S., Kwaaitaal, M., Kato, N. et al. Requirement of vesicle-associated membrane protein 721 and 722 for sustained growth during immune responses in Arabidopsis. Mol Cells 35, 481–488 (2013). https://doi.org/10.1007/s10059-013-2130-2

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  • DOI: https://doi.org/10.1007/s10059-013-2130-2

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