Culture-based methods for detection and identification of Streptococcus agalactiae in pregnant women—what are we missing?

Abstract

We aimed to compare BD group B Streptococcus differential agar (GBSDA) with inoculation into LIM broth and subculture onto blood agar plates (LIM-BA) as a method for GBS screening in pregnant women. First, we compared the detection threshold and the ease of use of GBSDA with LIM-BA by inoculating known numbers of GBS mixed with approximately 10⁵ cfu of other bacteria. Second, we tested the production of carotenoid pigment in 155 GBS blood culture isolates. Finally, we compared GBSDA, LIM broth with direct GBS antigen detection (LIM-AG) and LIM-BA as methods for GBS screening in pregnant women. GBS colonies were easily detected on GBSDA at a threshold of 20 cfu. In contrast, GBS was not detected in a mixed culture from LIM broth with initial inocula of up to 100 cfu. Orange pigment was produced in 146/155 GBS blood culture isolates. Pigment was not produced in eight non-hemolytic and two hemolytic strains. GBS was detected by GBSDA in 58 out of 297 parturient women (19.5%) but in only 50 (16.8%, P > 0.05) and 46 (15.4%, P = 0.079) women by LIM-BA and LIM-AG, respectively. GBSDA is a satisfactory medium for the rapid detection of most GBS isolates, but other methods may be needed if detection of all non-pigmented strains is required.