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Carbon dioxide laser and bonding materials reduce enamel demineralization around orthodontic brackets

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Abstract

Altering the structure of the enamel surface around the orthodontic bracket by reducing its content of carbonate and phosphate resulting from application of CO2 laser may represent a more effective strategy in preventing caries in this region. This study aimed at determining whether irradiation with a CO2 laser combined with fluoride-releasing bonding material could reduce enamel demineralization around orthodontic brackets subjected to cariogenic challenge. Ninety bovine enamel slabs were divided into five groups (n = 18): non-inoculated brain–heart infusion broth group, non-fluoride-releasing composite resin (NFRCR—control group), resin-modified glass ionomer cement (RMGIC), CO2 laser + Transbond (L+NFRCR) and CO2 laser + Fuji (L+RMGIC). Slabs were submitted to a 5-day microbiological caries model. The Streptococcus mutans biofilm formed on the slabs was biochemically and microbiologically analysed, and the enamel Knoop hardness number (KHN) around the brackets was determined. The data were analysed by ANOVA and Tukey tests (α = 0.05). Biochemical and microbiological analyses of the biofilm revealed no statistically significant differences among the groups. Lased groups presented the highest KHN means, which statistically differed from NFRCR; however, no difference was found between these lased groups. RMGIC did not differ from NFRCR which presented the lowest KHN mean. The CO2 laser (λ = 10.6 μm; 10.0 J/cm2 per pulse) use with or without F-bonding materials was effective in inhibiting demineralization around orthodontic brackets. However, no additional effect was found when the enamel was treated with the combination of CO2 laser and an F-releasing material.

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Acknowledgments

This study was supported by FAPESP (2008/02813-0) grant. The funder had no role in the study design, data collection and analysis, decision to publish or preparation of the manuscript. This paper was based on a thesis submitted by the first author to the Piracicaba Dental School, University of Campinas, in partial fulfillment of the requirements for a MS degree in Dentistry (Pediatric Dentistry area). The authors thank the Biochemistry Laboratory of FOBUSP and LELO-FOUSP Laboratory for the use of their equipments.

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Authors and Affiliations

  1. UNICAMP, Piracicaba Dental School, Campinas State University, São Paulo, Brazil

    Cíntia Maria de Souza-e-Silva, Thaís Manzano Parisotto, Carolina Steiner-Oliveira & Marinês Nobre-dos-Santos

  2. Federal University of Alagoas, Maceió, Brazil

    Regianne Umeko Kamiya

  3. Faculty of Pharmacy Dentistry and Nursing, Federal University of Ceará, Fortaleza, Brazil

    Lidiany Karla Azevedo Rodrigues

  4. Department of Pediatric Dentistry—Piracicaba Dental School, State University of Campinas—UNICAMP, Av. Limeira 901, Piracicaba, São Paulo, 13414-903, Brazil

    Marinês Nobre-dos-Santos

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  1. Cíntia Maria de Souza-e-Silva
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  2. Thaís Manzano Parisotto
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  3. Carolina Steiner-Oliveira
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Correspondence to Marinês Nobre-dos-Santos.

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de Souza-e-Silva, C.M., Parisotto, T.M., Steiner-Oliveira, C. et al. Carbon dioxide laser and bonding materials reduce enamel demineralization around orthodontic brackets. Lasers Med Sci 28, 111–118 (2013). https://doi.org/10.1007/s10103-012-1076-5

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