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1-(3′,5′-dihydroxyphenoxy)-7-(2″,4″,6-trihydroxyphenoxy)-2,4,9-trihydroxydibenzo-1,4-dioxin Inhibits Adipocyte Differentiation of 3T3-L1 Fibroblasts

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Abstract

In this study, we isolated the phloroglucinol derivative, 1-(3′,5′-dihydroxyphenoxy)-7-(2″,4″,6-trihydroxyphenoxy)-2,4,9-trihydroxydibenzo-1,4-dioxin (1), from Ecklonia cava and evaluated its potential inhibition on adipocyte differentiation in 3T3-L1 cells. Lipid accumulation along with the expression of several genes associated with adipogenesis and lipolysis was examined at the end of differentiation. Lipid accumulation level was examined by measuring triglyceride content and Oil-Red O staining. The expression levels of several genes and proteins were examined using reverse-transcription polymerase chain reaction (RT-PCR), real-time RT-PCR, and Western blot analysis. Compound 1 significantly reduced lipid accumulation and downregulated peroxisome proliferator-activated receptor-γ, sterol regulatory element-binding protein 1c, and CCAAT/enhancer-binding proteins α in a dose-dependent manner. Moreover, the presence of compound 1 induced downregulation of adipogenic target genes such as fatty acid binding protein 4, fatty acid transport protein 1, fatty acid synthase, acyl-CoA synthetase 1, lipoprotein lipase, and leptin. According to the lipolytic response, compound 1 downregulated perilipin and hormone-sensitive lipase while upregulating tumor necrosis factor alpha. Therefore, these results suggest that compound 1 might decrease lipid accumulation during adipocyte differentiation by modulating adipogenesis and lipogenesis. Furthermore, compound 1 could be developed as a functional agent effective in improving obesity.

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Abbreviations

ACS1:

Acyl-CoA synthetase 1

C/EBPα:

CCAAT/enhancer-binding proteins

DMEM:

Dulbecco’s modified Eagle medium

FABP:

Fatty acid binding protein

FAS:

Fatty acid synthase

FATP:

Fatty acid transport protein

FBS:

Fetal bovine serum

HSL:

Hormone-sensitive lipase

LPL:

Lipoprotein lipase

PBS:

Phosphate-buffered saline

PPARγ:

Peroxisome proliferator-activated receptor-γ

RT-PCR:

Reverse-transcription polymerase chain reaction

SREBP1c:

Sterol regulatory element-binding protein 1c

TNF-α:

Tumor necrosis factor alpha

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Acknowledgement

This research was supported by a grant from Marine Bioprocess Research Center of the Marine Biotechnology Program funded by the Ministry of Land, Transport, and Maritime, Republic of Korea.

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Authors and Affiliations

  1. Marine Bioprocess Research Center, Pukyong National University, Busan, 608-737, South Korea

    Chang-Suk Kong, Na-Young Yoon & Se-Kwon Kim

  2. Department of Chemistry, Pukyong National University, Busan, 608-737, South Korea

    Jung-Ae Kim, Byul-Nim Ahn, Thanh Sang Vo & Se-Kwon Kim

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  1. Chang-Suk Kong
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Correspondence to Se-Kwon Kim.

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Kong, CS., Kim, JA., Ahn, BN. et al. 1-(3′,5′-dihydroxyphenoxy)-7-(2″,4″,6-trihydroxyphenoxy)-2,4,9-trihydroxydibenzo-1,4-dioxin Inhibits Adipocyte Differentiation of 3T3-L1 Fibroblasts. Mar Biotechnol 12, 299–307 (2010). https://doi.org/10.1007/s10126-009-9224-z

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