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Chemical Derivatization of Metabolite Mass Profiling of the Recretohalophyte Aeluropus lagopoides Revealing Salt Stress Tolerance Mechanism

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Abstract

Plants are the primary producers of food for human being. Their intracellular environment alternation is influenced by abiotic stress factors such as drought, heat and soil salinity. Aeluropus lagopoides is a strong halophyte that grows with ease under high saline muddy banks of creeks of Gujarat, India. To study the response of salinity on metabolite changes in Aeluropus, three treatments, i.e. control, salinity and recovery, were selected for both shoot and root tissue. The cytosolic metabolite state was analysed by molecular chemical derivatization gas chromatography mass profiling. During saline treatment, significant increase of compatible solutes in shoot and root tissue was observed as compared to control. Subsequently, metabolic concentration decreased under recovery conditions. The metabolites like amino acids, organic acids and polyols were significantly detected in both shoot and root of Aeluropus under salinity. The metabolites like proline, aspartic acid, glycine, succinic acid and glycolic acid were significantly upregulated under stress. The salicylic acid was found to play a role in maintaining the polyols level by its down-regulation during salinity. The principle component analysis of all detected metabolites in both shoot and root showed that metabolites expressed under salinity (component 1) were highly variable, while metabolites expressed under recovery (component 2) were comparatively less variable as compared to control. The evolved intracellular compartmentalization of amino acids, organic acids and polyols in A. lagopoides can be a hallmark to sustaining at high salinity stress.

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Acknowledgements

CSIR-CSMCRI Communication No.-171/2016 (as provided by BDIM). MKP, PA and PM acknowledge the financial support from CSIR-networking project, DST-WOS-A scheme and DST-INSPIRE fellowship.

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Authors and Affiliations

  1. Plant Omics Division, CSIR-Central Salt and Marine Chemicals Research Institute (CSIR-CSMCRI), Council of Scientific and Industrial Research (CSIR), Gijubhai Badheka Marg, Bhavnagar, Gujarat, 364 002, India

    Murali Krishna Paidi, Parinita Agarwal, Prashant More & Pradeep K. Agarwal

  2. Academy of Scientific and Innovative Research, CSIR-Central Salt and Marine Chemicals Research Institute (CSIR-CSMCRI), Council of Scientific and Industrial Research (CSIR), Gijubhai Badheka Marg, Bhavnagar, Gujarat, 364 002, India

    Prashant More & Pradeep K. Agarwal

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  1. Murali Krishna Paidi
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  2. Parinita Agarwal
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Correspondence to Pradeep K. Agarwal.

Electronic supplementary material

Figure S1

Total ionic chromatograms of metabolites in shoot and root of Aeluropus lagopoides under control, salinity stress and recovery conditions. Chromatogam of each peak retention time, molecular mass, quantity and molecular NIST similarity library search results were represented in Supplementary Tables 1 and 2. (GIF 252 kb)

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Figure S2

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Table S1

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Table S2

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Table S3

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Table S4

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Paidi, M., Agarwal, P., More, P. et al. Chemical Derivatization of Metabolite Mass Profiling of the Recretohalophyte Aeluropus lagopoides Revealing Salt Stress Tolerance Mechanism. Mar Biotechnol 19, 207–218 (2017). https://doi.org/10.1007/s10126-017-9745-9

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