Abstract
To consolidate the genetic, physical, and cytogenetic maps of scallop (Patinopecten yessoensis), we constructed a molecular cytogenetic map by localizing 84 fosmid clones that contain different SNP markers from 19 linkage groups (LGs) using fluorescence in situ hybridization (FISH). Among these 84 SNP-anchored clones, 56 clones produced specific and stable signals on one pair of chromosomes. Dual-color FISH assigned 19 LGs to their corresponding chromosomes with 38 SNP-anchored clones as probes. Among these 19 LGs, 17 LGs were assigned to their corresponding one pair of chromosomes, while two clones containing SNPs from LG10 and LG19 were located on two different pairs of chromosomes separately. The orientation of 7 LGs was corrected according to the chromosome location of SNPs within the same LG. In addition, a probe panel of SNP-anchored clones was developed to identify each chromosome of P. yessoensis. The molecular cytogenetic map will facilitate molecular breeding in scallop and enable comparative studies on chromosome evolution of bivalve mollusk.
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Funding
This research was funded by the National Key R&D Program of China (2018YFD0901402 and 2018YFD0900304), the Earmarked Fund for Modern Agro-industry Technology Research System (CARS-49), and the Key Research and Development Program of Shandong Province (2016ZDJQ0208).
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Fig. S1
SNP-anchored clones covering 9 LGs (LG1, LG2, LG3, LG4, LG5, LG6, LG7, LG8, LG9) mapped on the single pairs of chromosomes. The arrow indicated red signals of clones correspond to digoxigenin-labeled probes located on specific chromosomal pairs. Scale bar = 10um. (PNG 618 kb)
Fig. S2
SNP-anchored clones covering 6 LGs (LG10, LG11, LG12, LG13, LG14, LG15) mapped on the single pairs of chromosomes. The arrow indicated red signals of clones correspond to digoxigenin-labeled probes located on specific chromosomal pairs. Scale bar = 10um. (PNG 335 kb)
Fig. S3
SNP-anchored clones covering 4 LGs (LG16, LG17, LG18, LG19) mapped on the single pairs of chromosomes. The arrow indicated red signals of clones correspond to digoxigenin-labeled probes located on specific chromosomal pairs. Scale bar = 10um. (PNG 340 kb)
Fig. S4
SNP-anchored clones did not produce any significant positive signals. a: the signals of clone distributed in multiple chromosomes. b: clones did not produce signals on chromosomes. c: the signals distributed in at least two pairs of chromosomes. Scale bar = 10um. (PNG 236 kb)
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Yang, Z., Li, X., Liao, H. et al. A Molecular Cytogenetic Map of Scallop (Patinopecten yessoensis). Mar Biotechnol 21, 731–742 (2019). https://doi.org/10.1007/s10126-019-09918-6
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DOI: https://doi.org/10.1007/s10126-019-09918-6