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Transcriptome analysis of Brassica juncea var. tumida Tsen responses to Plasmodiophora brassicae primed by the biocontrol strain Zhihengliuella aestuarii

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Abstract

Mustard clubroot, caused by Plasmodiophora brassicae, is a serious disease that affects Brassica juncea var. tumida Tsen, a mustard plant that is the raw material for a traditional fermented food manufactured in Chongqing, China. In our laboratory, we screened the antagonistic bacteria Zhihengliuella aestuarii against P. brassicae. To better understand the biocontrol mechanism, three transcriptome analyses of B. juncea var. tumida Tsen were conducted using Illumina HiSeq 4000, one from B. juncea only inoculated with P. brassicae (P), one inoculated with P. brassica and the biocontrol agent Z. aestuarii at the same time (P + B), and the other was the control (H), in which P. brassicae was replaced by sterile water. A total of 19.94 Gb was generated by Illumina HiSeq sequencing. The sequence data were de novo assembled, and 107,617 unigenes were obtained. In total, 5629 differentially expressed genes between biocontrol-treated (P + B) and infected (P) samples were assigned to 126 KEGG pathways. Using multiple testing corrections, 20 pathways were significantly enriched with Qvalue ≤ 0.05. The resistance-related genes, involved in the production of pathogenesis-related proteins, pathogen-associated molecular pattern-triggered immunity, and effector-triggered immunity signaling pathways, calcium influx, salicylic acid pathway, reactive oxygen intermediates, and mitogen-activated protein kinase cascades, and cell wall modification, were obtained. The various defense responses induced by the biocontrol strain combatted the P. brassicae infection. The genes and pathways involved in plant resistance were induced by a biocontrol strain. The transcriptome data explained the molecular mechanism of the potential biocontrol strain against P. brassicae. The data will also serve as an important public information platform to study B. juncea var. tumida Tsen and will be useful for breeding mustard plants resistant to P. brassicae.

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Acknowledgements

This work was financially supported by the Natural Science Foundation of Chongqing’s Science and Technology Commission (grant number: cstc 2013jcyjA80038) and the Special Fund for Post-Doctoral Research Project of Chongqing (grant number: Xm2015046).

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Authors and Affiliations

  1. Citrus Research Institute of Chinese Academy of Agricultural Sciences, Southwest University, Chongqing, 400712, People’s Republic of China

    Yuanli Luo & Changyong Zhou

  2. Southeast Chongqing Academy of Agricultural Sciences, Chongqing, 408000, People’s Republic of China

    Yuanli Luo, Daiwen Dong, Yu Su, Xuyi Wang, Yumei Peng & Jiang Peng

  3. Bioengineering College of Chongqing University, Key Lab of Gene Function and Regulation at Chongqing, Chongqing, 40030, People’s Republic of China

    Yu Su

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  1. Yuanli Luo
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Contributions

CYZ and YLL conceived and designed the study. YLL, DWD, YS, XYW, YMP, and JP collected samples and performed the experiment. YLL carried out the data analysis. YLL and CYZ contributed to the writing of the manuscript. All of the authors read and approved the final manuscript.

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Correspondence to Changyong Zhou.

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Luo, Y., Dong, D., Su, Y. et al. Transcriptome analysis of Brassica juncea var. tumida Tsen responses to Plasmodiophora brassicae primed by the biocontrol strain Zhihengliuella aestuarii. Funct Integr Genomics 18, 301–314 (2018). https://doi.org/10.1007/s10142-018-0593-0

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