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Community of arbuscular mycorrhizal fungi in drought-resistant plants, Moringa spp., in semiarid regions in Madagascar and Uganda

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Mycoscience

Abstract

The community structure of arbuscular mycorrhizal (AM) fungi in the roots of drought-resistant trees, Moringa spp., was examined in semiarid regions in Madagascar and Uganda. Root samples were collected from 8 individuals of M. hildebrandtii and 2 individuals of M. drouhardii in Madagascar and from 21 individuals of M. oleifera in Uganda. Total DNA was extracted from the root samples, and partial nSSU rDNA of AM fungi was amplified using a universal eukaryotic primer NS31 and an AM fungalspecific primer AM1. The PCR products were cloned and divided by restriction fragment length polymorphism (RFLP) analysis with HinfI and RsaI. Some representatives in each RFLP types were sequenced, and a neighbor-joining phylogenetic analysis was conducted for the obtained sequences with analogous sequences of AM fungi. The RFLP and phylogenetic analyses showed that AM fungi closely related to Glomus intraradices or G. sinuosum were detected in many samples. The AM fungal groups frequently detected in the Moringa spp. might be widely distributed species in semiarid environments.

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Authors and Affiliations

  1. Environment Department, The General Environmental Technos, Co., Ltd., 1-3-5 Azuchimachi, Chuou-ku, Osaka, 541-0052, Japan

    Masahide Yamato

  2. Forest Development Technological Institute, Kyoto, Japan

    Shiho Ikeda

  3. Fungus/Mushroom Resource and Research Center, Faculty of Agriculture, Tottori University, Tottori, Japan

    Koji Iwase

Authors
  1. Masahide Yamato
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  2. Shiho Ikeda
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  3. Koji Iwase
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Correspondence to Masahide Yamato.

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Yamato, M., Ikeda, S. & Iwase, K. Community of arbuscular mycorrhizal fungi in drought-resistant plants, Moringa spp., in semiarid regions in Madagascar and Uganda. Mycoscience 50, 100–105 (2009). https://doi.org/10.1007/s10267-008-0459-8

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  • DOI: https://doi.org/10.1007/s10267-008-0459-8

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