Abstract
Brevipalpus-transmitted viruses (BTV) cause chlorotic, necrotic and/or ringspot lesions in leaves and stems of orchids, citrus, coffee and several other plant species. There are two different types of BTVs, the nuclear and the cytoplasmic, based on maturation locale in the cell and particle morphology. The orchid fleck virus (OFV) is a BTV that infects orchids. Its short rodlike particles are 32–40 nm in diameter, 100–150 nm in length. OFV is found in the nucleus and is associated with intranuclear electronlucent viroplasms. In 1999, transmission electron microscopy analysis revealed a distinct type of virus causing orchid fleck symptoms. The bacilliform particles, 70–80 nm in diameter and 110–120 nm in length, induced electron-dense viroplasm inclusions in infected cells and resembled the cytoplasmic type associated with BTV, such as the citrus leprosis virus C. Our objective in the present study was to verify whether the cytoplasmic type virus found in orchids could be amplified using primers for other cytoplasmic BTVs, such as CiLV-C and Solanum violaefolium ringspot virus (SvRSV). Additionally, we aimed to differentiate the two BTVs found in orchids: the nuclear and the cytoplasmic types of OFV using microscopy and molecular and serological tools. This virus was not amplified by the CiLV-C and SvRSV primers, and neither the molecular nor the serological tools available to the OFV diagnosis reacted with it, demonstrating that they are definitely different viruses.
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Acknowledgments
This work was part of the MSc thesis (Plant Pathology, Universidade de São Paulo) of the senior author. The authors would like to express their gratitude to Dr. Hideki Kondo (Research Institute for Bioresources, Okayama University, Japan) for providing OFV antiserum to FAPESP for a scholarship to the first author and to FAPESP and EMBRAPA for partially funding this work.
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Kubo, K.S., Freitas-Astúa, J., Machado, M.A. et al. Orchid fleck symptoms may be caused naturally by two different viruses transmitted by Brevipalpus . J Gen Plant Pathol 75, 250–255 (2009). https://doi.org/10.1007/s10327-009-0167-z
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DOI: https://doi.org/10.1007/s10327-009-0167-z