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Seed dormancy and germination of a critically endangered plant, Elaeagnus mollis, on the Loess Plateau of China

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Abstract

Elaeagnus mollis is an endangered species narrowly distributed on the south-eastern Loess Plateau of China. Natural regeneration of populations is highly restricted by low seed germination. The aim of this study was to explore why germination is low and how to enhance germination of the species. Field and laboratory experiments were carried out to examine the effects of temperature, light, drought and NaCl on seed germination and the effects of the seed coat, GA3, cold and warm stratification and after-ripening on dormancy release. Our results showed that (1) fruits (0.65 g/fruit) and seeds (0.14 g/seed) were relatively large, the embryo was surrounded by a hard and permeable seed coat and a lignified calyx tube, and more than half of the fresh seeds were not viable; (2) fresh, intact seeds did not germinate, but germination of scarified seeds was highest at 5/15 °C in continuous darkness; (3) dormancy was not released by GA3 and cold stratification (5 °C), but significantly released by variable temperature stratification and field burial, indicating that seeds had deep physiological dormancy; and (4) germination was significantly enhanced by median drought (−0.6 MPa) and NaCl (0.34 M), and decay was inhibited beyond −0.4 MPa osmotic potential and 0.225 M NaCl. Our results suggested that, with habitat loss and climate warming, regeneration from seeds in natural populations will continue to be negatively affected, and the range of the populations will further contract.

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Acknowledgements

We are grateful to Wenhua Zhang and Yanhong Li in the E. mollis Nature Reserve for help to collect seeds. This work was supported by National Natural Science Foundation of China [Grant Numbers 31770514, 31470476].

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Authors and Affiliations

  1. The School of Life Sciences, Shanxi Normal University, Linfen, 041000, China

    Ruiru Gao, Jing Hou, Ruihua Zhao, Xinyu Hou & Liping Huo

  2. State Key Laboratory of Vegetation and Environmental Change, Institute of Botany, Chinese Academy of Sciences, Beijing, 100049, China

    Xuejun Yang

  3. Department of Biology, Middle Tennessee State University, Murfreesboro, TN, 37132, USA

    Siti N. Hidayati & Jeffrey L. Walck

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  1. Ruiru Gao
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  2. Jing Hou
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  8. Jeffrey L. Walck
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Correspondence to Ruiru Gao or Xuejun Yang.

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Communicated by Gediminas Brazaitis.

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Gao, R., Hou, J., Zhao, R. et al. Seed dormancy and germination of a critically endangered plant, Elaeagnus mollis, on the Loess Plateau of China. Eur J Forest Res 140, 451–461 (2021). https://doi.org/10.1007/s10342-020-01342-z

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