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Influence of Nitric Oxide Application on Some Biochemical Aspects, Endogenous Hormones, Minerals and Phenolic Compounds of Vicia faba Plant Grown under Arsenic Stress

Einfluss von Stickoxidanwendung auf einige biochemische Aspekte, endogene Hormone, Mineralien und Phenolverbindungen von Vicia faba-Pflanzen, die unter Arsenbelastung heranwachsen

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Abstract

Arsenic is a non-essential and toxic heavy metal, which can cause physiological disorder in plants. The present study was undertaken to examine the possible roles of sodium nitroprusside (SNP, a donor of NO) in protection against oxidative damage due to arsenic (As) toxicity in faba bean (Vicia faba L.) plants. In this study, we measured physiochemical parameters (photosynthetic pigments, lipid peroxidation, endogenous hormones, minerals and phenolic contents) in faba bean plants exposed to AS (100, 200 and 400 µM) alone or combined with NO (100 µM). The results showed that application of all concentrations of arsenic (100, 200 and 400 µM) induced decrease on growth parameters, seed yield, photosynthetic pigments, phytohormones, minerals contents (N3−, P3−, K+, Ca2+ and Mg2+) as compared to control plants but increased lipid peroxidation, Na + and total phenolic compounds fraction. Foliar application of 100 µM NO reversed the inhibition induced by As treatment. In addition, arsenic was undetected in harvested seeds at all applied concentrations of arsenic. Therefore, it could be concluded that NO might account for the alleviating effect of As stress on Vicia faba plants.

Zusammenfassung

Arsen ist ein nicht-essentielles und giftiges Schwermetall, das in Pflanzen zu physiologischen Störungen führen kann. Die vorliegende Studie wurde durchgeführt, um die möglichen Rollen von Natriumnitroprussid (Sodium Nitroprusside = SNP, ein NO-Ableger) als Schutz gegen oxidative Schädigung aufgrund von Arsen-Toxizität (As) in Puffbohnen-Pflanzen (Vicia faba L.) zu untersuchen. In dieser Studie ermittelten wir physiochemische Parameter (photosynthetische Pigmente, Lipidperoxidation, endogene Hormone, Mineralien und Phenolgehalt) in Puffbohnen-Pflanzen, die AS (100, 200 and 400 µM) allein oder kombiniert mit NO (100 µM) ausgesetzt waren. Die Ereignisse zeigten, dass verglichen zu den Kontrollpflanzen, die Anwendung aller Konzentrationen von Arsen (100, 200 and 400 µM) zu einem Abfall der Wachstumsparameter, Samenernte, photosynthetischen Pigmente, Phytohormone und des Mineraliengehalts (N3−, P3−, K+, Ca2+ and Mg2+) führte; es gab jedoch erhöhte Werte für Lipidperoxidation, Na+ und den Gesamtanteil an Phenolverbindungen. Die Blattanwendung von 100 µM NO kehrte die durch die As-Behandlung induzierte Inhibierung um. Zusätzlich wurde Arsen bei allen angewendeten Arsenkonzentrationen nicht in geernteten Samen entdeckt. Daher konnte geschlussfolgert werden, dass NO der Grund für den mildernden Effekt bei der As-Belastung von Vicia faba-Pflanzen ist.

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Acknowledgment

We wish to express my deep thanks and gratitude to Prof. Dr. Awatif Aly Mohsen, Professor of Plant Physiology, Plant Department, Faculty of Sciences, Tanta University, for critical review of this manuscript and continuous encouragement throughout this work.

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  1. Biological and Geological Sciences Department, Faculty of Education, Ain Shams University, 1575, Cairo, Egypt

    Heba Ibrahim Mohamed, Hanan H. Latif & Rania S. Hanafy

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Correspondence to Heba Ibrahim Mohamed.

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Mohamed, H.I., Latif, H.H. & Hanafy, R.S. Influence of Nitric Oxide Application on Some Biochemical Aspects, Endogenous Hormones, Minerals and Phenolic Compounds of Vicia faba Plant Grown under Arsenic Stress. Gesunde Pflanzen 68, 99–107 (2016). https://doi.org/10.1007/s10343-016-0363-7

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