Abstract
An AC electroosmotic (AC-EO) microconcentrator using a face-to-face, asymmetric electrode pair with expanded sections in the bottom electrode is proposed in this study. The electrode pair of the AC-EO microconcentrator is composed of a larger top electrode (30 mm × 60 mm) and a bottom electrode (containing three slim electrodes and a triangular electrode). In the expanded section at the connection of a slim electrode and the triangular electrode, an electroosmosis flow transports test samples far away from the triangular electrode to the stagnation zone inside the triangular electrode through the slim electrode for concentration. On the three sides of the triangular electrode, vortices bring test samples surrounding the triangular electrode to the stagnation zone. By these two electroosmosis flow fields, the microconcentrator can concentrate test samples near and far from the triangular electrode to its central area, achieving a highly efficient sample concentration. The measured concentration distribution in the vertical electrode direction by confocal microscopy indicates that the concentration process occurs above the electrode surface. The capability of the proposed AC-EO concentrator in the repeated concentration and release of test samples is verified by a reversible switch test. The performance of the proposed AC-EO concentrator in concentrating latex particles and T4 GT7 DNA is better than those reported in the literature under similar average electric field strength. The fluorescence enhancement factor is 3.9–9.1 times better when concentrating latex particles, and the concentration enhance factor is 1.4–5.7 times better when concentrating T4 GT7 DNA.
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Bashir M, Mahara JB, Torkos K (1998) Liquid–liquid extraction for sample preparation prior to gas chromatography and gas chromatography–mass spectrometry determination of herbicide and pesticide compounds. Microchem J 58:31–38
Bown MR, Meinhart CD (2006) AC electroosmotic flow in a DNA concentrator. Microfluid Nanofluid 2:513–523
Broyles BS, Jacobson SC, Ramsey JM (2003) Sample filtration, concentration, and separation integrated on microfluidic devices. Anal Chem 75:2761–2767
Chen DF, Du HJ (2010) A microfluidic device for rapid concentration of particles in continuous flow by DC dielectrophoresis. Microfluid Nanofluid 9:281–291. doi:10.1007/s10404-009-0545-z
Chen DF, Du HJ, Tay CY (2010a) Rapid concentration of nanoparticles with DC dielectrophoresis in focused electric fields. Nanoscale Res Lett 5:55–60. doi:10.1007/s11671-009-9442-3
Chen JL, Shih WH, Hsieh WH (2010b) Three-dimensional non-linear AC electro-osmotic flow induced by a face-to-face, asymmetric pair of planar electrodes. Microfluid Nanofluid 9:579–584. doi:10.1007/s10404-010-0582-7
Chiou PY, Ohta AT, Jamshidi A, Hsu HY, Wu MC (2008) Light-actuated ac electroosmosis for nanoparticle manipulation. J Microelectromech Syst 17:525–531
Cho YK, Kim S, Lee K, Park C, Lee JG, Ko C (2009) Bacteria concentration using a membrane type insulator-based dielectrophoresis in a plastic chip. Electrophoresis 30:3153–3159. doi:10.1002/elps.200900179
Cho YK, Kim TH, Lee JG (2010) ) On-chip concentration of bacteria using a 3D dielectrophoretic chip and subsequent laser-based DNA extraction in the same chip. J Micromech Microeng. doi:10.1088/0960-1317/20/6/065010
Cummings EB, Simmons BA, Davalos RV, McGraw GJ, Lapizco-Encinas BH, Fintschenko Y (2005) Fast and selective concentration of pathogens by insulator based dielectrophoresis. Abstr Pap Am Chem Soc 230:U404–U405
Dimeric Cyanine Nucleic Acid Stains, Product Information. Molecular Probes. https://tools.thermofisher.com/content/sfs/manuals/mp03600.pdf
Du JR, Wei HH (2010) Focusing and trapping of DNA molecules by head-on ac electrokinetic streaming through join asymmetric polarization. Biomicrofluidics 4, 034108
Du JR, Juang YJ, Wu JT, Wei HH (2008) Long-range and superfast trapping of DNA molecules in an ac electrokinetic funnel. Biomicrofluidics 2, 044103
Gencoglu A, Olney D, LaLonde A, Koppula KS, Lapizco-Encinas BH (2014) Dynamic microparticle manipulation with an electroosmotic flow gradient in low-frequency alternating current dielectrophoresis. Electrophoresis 35:362–373. doi:10.1002/elps.201300385
Ghubade A, Mandal S, Chaudhury R, Singh RK, Bhattacharya S (2009) Dielectrophoresis assisted concentration of micro-particles and their rapid quantitation based on optical means. Biomed Microdevices 11:987–995. doi:10.1007/s10544-009-9316-6
Giordano BC, Burgi DS, Hart SJ, Terray A (2012) On-line sample pre-concentration in microfluidic devices: a review. Anal Chim Acta 718:11–24
Gutzman Y, Carroll AD, Ruzicka J (2006) Bead injection for biomolecular assays: affinity chromatography enhanced by bead injection spectroscopy. Analyst 131:809–815
Hansang C, Lee LP (2006) A novel integrated microfluidic SERS-CD with high-throughput centrifugal cell trapping array for quantitative biomedicine. In: Society for chemistry and micro-nano systems, pp 5–9
Hayashi M, Yasuda K (2010) Simple microfluidic continuous concentration of microparticles with different dielectric constants using dielectrophoretic force in a V-shaped electrode array. Jpn J Appl Phys. doi:10.1143/Jjap.49.097002
Hayashi M, Kaneko T, Yasuda K (2011) Continuous concentration and separation of microparticles using dielectrophoretic force in a V-shaped electrode array. Jpn J Appl Phys. doi:10.1143/Jjap.50.06gl03
Henslee EA, Sano MB, Rojas AD, Schmelz EM, Davalos RV (2011) Selective concentration of human cancer cells using contactless dielectrophoresis. Electrophoresis 32:2523–2529. doi:10.1002/elps.201100081
Hoettges KF, McDonnell MB, Hughes MP (2014) Continuous flow nanoparticle concentration using alternating current-electroosmotic flow. Electrophoresis 35:467–473. doi:10.1002/elps.201300287
ImageJ home page. NIH, National Institutes of Health. http://rsbweb.nih.gov/ij/
Islam N, Lian M, Wu J (2007) Enhancing microcantilever capability with integrated AC electroosmotic trapping. Microfluid Nanofluid 3:369–375
Lagally ET, Lee SH, Soh HT (2005) Integrated microsystem for dielectrophoretic cell concentration and genetic detection. Lab Chip 5:1053–1058. doi:10.1039/B505915a
Lapizco-Encinas BH, Simmons BA, Cummings EB, Fintschenko Y (2004a) Dielectrophoretic concentration and separation of live and dead bacteria in an array of insulators. Anal Chem 76:1571–1579. doi:10.1021/Ac034804j
Lapizco-Encinas BH, Simmons BA, Cummings EB, Fintschenko Y (2004b) Insulator-based dielectrophoresis for the selective concentration and separation of live bacteria in water. Electrophoresis 25:1695–1704. doi:10.1002/elps.200405899
Lapizco-Encinas BH, Davalos RV, Simmons BA, Cummings EB, Fintschenko Y (2005) An insulator-based (electrodeless) dielectrophoretic concentrator for microbes in water. J Microbiol Methods 62:317–326. doi:10.1016/j.mimet.2005.04.027
Lei KF, Cheng H, Choy KY, Chow LMC (2009) Electrokinetic DNA concentration in microsystems. Sensor Actuat A Phys 156:381–387
Lewpiriyawong N, Yang C, Lam YC (2012) Electrokinetically driven concentration of particles and cells by dielectrophoresis with DC-offset AC electric field. Microfluid Nanofluid 12:723–733. doi:10.1007/s10404-011-0919-x
Li M, Li SB, Cao WB, Li WH, Wen WJ, Alici G (2013a) Improved concentration and separation of particles in a 3D dielectrophoretic chip integrating focusing, aligning and trapping. Microfluid Nanofluid 14:527–539. doi:10.1007/s10404-012-1071-y
Li SB, Li M, Bougot-Robin K, Cao WB, Chau IYY, Li WH, Wen WJ (2013b) High-throughput particle manipulation by hydrodynamic, electrokinetic, and dielectrophoretic effects in an integrated microfluidic chip. Biomicrofluidics. doi:10.1063/1.4795856
Martinez-Lopez JI, Moncada-Hernandez H, Baylon-Cardiel JL, Martinez-Chapa SO, Rito-Palomares M, Lapizco-Encinas BH (2009) Characterization of electrokinetic mobility of microparticles in order to improve dielectrophoretic concentration. Anal Bioanal Chem 394:293–302. doi:10.1007/s00216-009-2626-y
Maruyama H, Kotani K, Masuda T, Honda A, Takahata T, Arai F (2011) Nanomanipulation of single influenza virus using dielectrophoretic concentration and optical tweezers for single virus infection to a specific cell on a microfluidic chip. Microfluid Nanofluid 10:1109–1117. doi:10.1007/s10404-010-0739-4
Moncada-Hernandez H, Lapizco-Encinas BH (2010) Simultaneous concentration and separation of microorganisms: insulator-based dielectrophoretic approach. Anal Bioanal Chem 396:1805–1816. doi:10.1007/s00216-009-3422-4
Motosuke M, Yamasaki K, Ishida A, Toki H, Honami S (2013) Improved particle concentration by cascade AC electroosmotic flow. Microfluid Nanofluid 14:1021–1030. doi:10.1007/s10404-012-1109-1
Park S, Zhang Y, Wang TH, Yang S (2011) Continuous dielectrophoretic bacterial separation and concentration from physiological media of high conductivity. Lab Chip 11:2893–2900. doi:10.1039/C1lc20307j
Puttaswamy SV, Lin CH, Sivashankar S, Yang YS, Liu CH (2013) Electrodeless dielectrophoretic concentrator for analyte pre-concentration on poly-silicon nanowire field effect transistor. Sensor Actuat B Chem 178:547–554. doi:10.1016/j.snb.2013.01.016
Shihabi Z (2006) Review: sample concentration based on inclusion of organic solvents in capillary zone electrophoresis. Curr Pharm Anal 2:9–15. doi:10.2174/157341206775474025
Sin MLY, Gau V, Liao JC, Haake DA, Wong PK (2009) Active manipulation of quantum dots using AC electrokinetics. J Phys Chem C 113:6561–6565
Wu JT, Du JR, Juang YJ, Wei HH (2007) Rectified elongational streaming due to asymmetric electro-osmosis induced by ac polarization. Appl Phys Lett 90, 134103
Xu Y, Cao Q, Zeng X, Wu YJ, Zhang WP (2009) Research of cell concentration and separation on the dielectrophoretic chip with arrayed opposite electrodes. Chem J Chin Univ 30:876–881
Yamashita K, Yamaguchi Y, Miyazaki M, Nakamura H, Shimizu H, Maeda H (2004) Direct observation of long-strand DNA conformational changing in microchannel flow and microfluidic hybridization assay. Anal Biochem 332:274–279. doi:10.1016/j.ab.2004.05.040
Yang LJ, Banada PP, Chatni MR, Lim KS, Bhunia AK, Ladisch M, Bashir R (2006) A multifunctional micro-fluidic system for dielectrophoretic concentration coupled with immuno-capture of low numbers of Listeria monocytogenes. Lab Chip 6:896–905. doi:10.1039/B607061m
Yeo WH, Chung JH, Liu YL, Lee KH (2009) Size-specific concentration of DNA to a nanostructured tip using dielectrophoresis and capillary action. J Phys Chem B 113:10849–10858. doi:10.1021/Jp900618t
Yeo WH et al (2012) Dielectrophoretic concentration of low-abundance nanoparticles using a nanostructured tip. Nanotechnology. doi:10.1088/0957-4484/23/48/485707
Yeo WH, Lee HB, Kim JH, Lee KH, Chung JH (2013) Nanotip analysis for dielectrophoretic concentration of nanosized viral particles. Nanotechnology 24:1855. doi:10.1088/0957-4484/24/18/185502
Yokokawa R, Manta Y, Namura M, Takizawa Y, Le NCH, Sugiyama S (2010) Individual evaluation of DEP, EP and AC-EOF effects on lambda DNA molecules in a DNA concentrator. Sensor Actuat B Chem 143:769–775
Zambonin CG, Losito I, Cilenti A, Palmisano F (2002) Solid-phase microextraction coupled to gas chromatography-mass spectrometry for the study of soil adsorption coefficients of organophosphorus pesticides. J Environ Monitor 4:477–481
Zhang W, Cao CX (2005) A review on stacking of analytes in high salt sample in capillary electrophoresis. Chin J Anal Chem 33:267–271
Zhao YJ, Yi UC, Cho SK (2007) Microparticle concentration and separation by traveling-wave dielectrophoresis (twDEP) for digital microfluidics. J Microelectromech Syst 16:1472–1481. doi:10.1109/Jmems.2007.906763
Zhou RH, Wang P, Chang HC (2006) Bacteria capture, concentration and detection by alternating current dielectrophoresis and self-assembly of dispersed single-wall carbon nanotubes. Electrophoresis 27:1376–1385. doi:10.1002/elps.200500329
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Chen, JH., Lee, YC. & Hsieh, WH. AC electroosmotic microconcentrator using a face-to-face, asymmetric electrode pair with expanded sections in the bottom electrode. Microfluid Nanofluid 20, 72 (2016). https://doi.org/10.1007/s10404-016-1736-z
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DOI: https://doi.org/10.1007/s10404-016-1736-z