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Roseomonas alkaliterrae sp. nov., isolated from an alkali geothermal soil sample in Tengchong, Yunnan, south-west China

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Abstract

An alkalitolerant, thermotolerant and Gram-stain negative bacterium, designated strain YIM 78007T, was isolated from an alkaline geothermal soil sample from Hehua hot spring, Tengchong, Yunnan province, south-west China. Cells of strain YIM 78007T were observed to be aerobic and short rod-shaped. The colonies were observed to be orange-red, convex and circular. 16S rRNA gene sequence-based phylogenetic analysis showed that strain YIM 78007T clustered with members of the genus Roseomonas (with similarities from 97.2 to 92.2 %). Optimal growth of strain YIM 78007 occurs at 40–50 °C and pH 8.0–10.0. The predominant ubiquinone was identified as Q-10 and the major fatty acids were identified as C18:1 ω7c and C16:0. The polar lipids were identified as diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine, two unidentified aminolipids and one unknown phospholipid. The G + C content of the genomic DNA was determined to be 63 mol %. The levels of DNA–DNA hybridization relatedness between strain YIM 78007T and its closet neighbours (Roseomonas lacus JCM 13283T and Roseomonas terrae JCM 14592T) were well below the threshold required for the proposal of a novel species. The results of physiological and biochemical characteristics, the phylogenetic analysis, as well as low DNA–DNA hybridization values, allowed the phenotypic and genotypic differentiation of strain YIM 78007T from its closest phylogenetic neighbours. Therefore, strain YIM 78007T is considered to represent a novel species of the genus Roseomonas, for which the name Roseomonas alkaliterrae sp. nov. is proposed. The type strain is YIM 78007T (=BCRC 80644T = JCM 19656T).

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Acknowledgments

The authors are grateful to Prof. Dr. Takuji Kudo (JCM, Japan) for his kindly providing reference type strains. This research was supported by the Key Project of International Cooperation of Ministry of Science & Technology (MOST) (No. 2013DFA31980), National Science Foundation Grant (OISE-0968421) and Key Project of Yunnan Provincial Natural Science Foundation (2013FA004).

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Authors and Affiliations

  1. Key Laboratory of Microbial Diversity in Southwest China, Ministry of Education, and Laboratory for Conservation and Utilization of Bio-resources, Yunnan Institute of Microbiology, Yunnan University, Kunming, 650091, People’s Republic of China

    Lei Dong, Hong Ming, Yi-Rui Yin, En-Min Zhou, Lan Liu & Wen-Jun Li

  2. College of Life Sciences and Technology, Xinxiang Medical University, Xinxiang, 453003, People’s Republic of China

    Hong Ming & Hui-Geng Feng

  3. College of Fisheries, Henan Normal University, Xinxiang, 453007, People’s Republic of China

    Yan-Yan Duan & Guo-Xing Nie

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  1. Lei Dong
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  2. Hong Ming
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  3. Yi-Rui Yin
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  9. Wen-Jun Li
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Correspondence to Wen-Jun Li.

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Lei Dong and Hong Ming are contributed equally to this work.

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Dong, L., Ming, H., Yin, YR. et al. Roseomonas alkaliterrae sp. nov., isolated from an alkali geothermal soil sample in Tengchong, Yunnan, south-west China. Antonie van Leeuwenhoek 105, 899–905 (2014). https://doi.org/10.1007/s10482-014-0144-1

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