Abstract
Two extremely halophilic archaeal strains GX1T and GX60 were isolated from the Gangxi marine solar saltern, China. Cells from the two strains were observed to be rod-shaped and stained Gram-negative, with red-pigmented colonies. Strains GX1T and GX60 were found to be able to grow at 25–50 °C (optimum 37 °C), at 1.4–4.8 M NaCl (optimum 2.6 M), at pH 5.5–9.5 (optimum pH 7.0) and neither strain required Mg2+ for growth. The cells lysed in distilled water and the minimal NaCl concentration to prevent cell-lysis was found to be 8 % (w/v). The major polar lipids of the two strains were identified as phosphatidic acid, phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester, phosphatidylglycerol sulfate and three glycolipids chromatographically identical to those of Haloarchaeobius iranensis IBRC-M 10013T. 16S rRNA gene analysis revealed that each strain had two dissimilar 16S rRNA genes and both strains were phylogenetically related to Hab. iranensis IBRC-M 10013T (94.9–98.9 % nucleotide identity). The rpoB′ gene similarity between strains GX1T and GX60, and between these strains and Hab. iranensis IBRC-M 10013T were found to be 99.6, 96.0 and 95.8 %, respectively. The DNA G + C content of strain GX1T and GX60 were determined to be 67.7 and 67.8 mol %, respectively. The DNA–DNA hybridization value of strains GX1T and GX60 was 86 % and the two strains showed low DNA–DNA relatedness with Hab. iranensis IBRC-M 10013T (38 and 32 %). It was concluded that strain GX1T (= CGMCC 1.10390T = JCM 17114T) and strain GX60 (= CGMCC 1.10389 = JCM 17120) represent a new species of Haloarchaeobius, for which the name Haloarchaeobius litoreus sp. nov. is proposed.
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Acknowledgments
This work was supported by the National Natural Science Foundation of China (No. 31370054), the grant from China Ocean Mineral Resources R & D Association (COMRA) Special Foundation (DY125-15-R-03), the Qinglan Project of Jiangsu Province and a project funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD). We are grateful to Dr. M. A. Amoozegar for kindly providing strain Haloarchaeobius iranensis IBRC-M 10013T.
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Zhang, WJ., Cui, HL. Haloarchaeobius litoreus sp. nov., isolated from a marine solar saltern. Antonie van Leeuwenhoek 105, 1085–1090 (2014). https://doi.org/10.1007/s10482-014-0166-8
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DOI: https://doi.org/10.1007/s10482-014-0166-8