Abstract
A Gram-stain positive, non-motile, rod-shaped bacterium, designated strain 1111S-42T, was isolated from the East Siberian Sea. The organism was found to grow at 4–30 °C, pH 7.0–8.5 and in 0–8 % (w/v) NaCl, with optimal growth occurring at 28 °C, pH 7.5 and in 1 % NaCl. Based on 16S rRNA gene sequence similarity studies, strain 1111S-42T was found to belong to the genus Sporosarcina and to be most closely related to Sporosarcina contaminans CCUG53915T (97.3 %) and Sporosarcina soli I80T (97.2 %). The main polar lipids were found to include diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine. The predominant menaquinone was identified as MK-7. The major cellular fatty acids were identified as anteiso-C15:0 (34.4 %), iso-C15:0 (29.8 %) and anteiso-C17:0 (22.4 %). The DNA G+C content of strain 1111S-42T was determined to be 39 mol %. The values of DNA–DNA relatedness between the strain 1111S-42T and related type strains of the genus Sporosarcina were less than 30 %. Based on the phylogenetic analysis, along with extensive physiological and chemotaxonomic testing, we conclude that the bacterium represents a novel species of the genus Sporosarcina, for which the name Sporosarcina siberiensis sp. nov. is proposed. The type strain is strain 1111S-42T (=CGMCC 1.12516T = LMG 27494T).
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Acknowledgments
This work was supported by grants from COMRA program (No. DY125-15-R-01), National Natural Science Foundation of China (41206160 and 312700040) and Scientific Research Foundation of Third Institute of Oceanography, SOA (2011002).
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Zhang, G., Ren, H., Chen, X. et al. Sporosarcina siberiensis sp. nov., isolated from the East Siberian Sea. Antonie van Leeuwenhoek 106, 489–495 (2014). https://doi.org/10.1007/s10482-014-0217-1
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DOI: https://doi.org/10.1007/s10482-014-0217-1