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d-Xylose fermentation, xylitol production and xylanase activities by seven new species of Sugiyamaella

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Abstract

Sixteen yeast isolates identified as belonging to the genus Sugiyamaella were studied in relation to D-xylose fermentation, xylitol production, and xylanase activities. The yeasts were recovered from rotting wood and sugarcane bagasse samples in different Brazilian regions. Sequence analyses of the internal transcribed spacer (ITS) region and the D1/D2 domains of large subunit rRNA gene showed that these isolates belong to seven new species. The species are described here as Sugiyamaella ayubii f.a., sp. nov. (UFMG-CM-Y607T = CBS 14108T), Sugiyamaella bahiana f.a., sp. nov. (UFMG-CM-Y304T = CBS 13474T), Sugiyamaella bonitensis f.a., sp. nov. (UFMG-CM-Y608T = CBS 14270T), Sugiyamaella carassensis f.a., sp. nov. (UFMG-CM-Y606T = CBS 14107T), Sugiyamaella ligni f.a., sp. nov. (UFMG-CM-Y295T = CBS 13482T), Sugiyamaella valenteae f.a., sp. nov. (UFMG-CM-Y609T = CBS 14109T) and Sugiyamaella xylolytica f.a., sp. nov. (UFMG-CM-Y348T = CBS 13493T). Strains of the described species S. boreocaroliniensis, S. lignohabitans, S. novakii and S. xylanicola, isolated from rotting wood of Brazilian ecosystems, were also compared for traits relevant to xylose metabolism. S. valenteae sp. nov., S. xylolytica sp. nov., S. bahiana sp. nov., S. bonitensis sp. nov., S. boreocarolinensis, S. lignohabitans and S. xylanicola were able to ferment d-xylose to ethanol. Xylitol production was observed for all Sugiyamaella species studied, except for S. ayubii sp. nov. All species studied showed xylanolytic activity, with S. xylanicola, S. lignohabitans and S. valenteae sp. nov. having the highest values. Our results suggest these Sugiyamaella species have good potential for biotechnological applications.

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Acknowledgments

This work was funded by Conselho Nacional de Desenvolvimento Cientifico e Tecnológico (CNPq—Brazil, Process Number 457499/2014-1), Fundação do Amparo a Pesquisa do Estado de Minas Gerais (FAPEMIG, process number APQ-01525-14) and the Natural Science and Engineering Research Council of Canada (MAL).

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    Authors and Affiliations

    1. Departamento de Microbiologia, ICB, C.P. 486, Universidade Federal de Minas Gerais, Belo Horizonte, MG, 31270-901, Brazil

      Letícia M. F. Sena, Camila G. Morais, Mariana R. Lopes, Renata O. Santos & Carlos A. Rosa

    2. Departamento de Ciências Biológicas e Agroindústria, Universidade Estadual Santa Cruz, Ilhéus, BA, 45662-900, Brazil

      Ana P. T. Uetanabaro

    3. Laboratorio de Microbiologia Ambiental e Biotecnologia, Universidade Federal do Tocantins, Palmas, TO, 77020-220, Brazil

      Paula B. Morais

    4. Centro de Estudos da Biodiversidade, Universidade Federal de Roraima, Campus do Paricarana, Boa Vista, Brazil

      Marcos J. S. Vital

    5. Interdepartmental Research Group in Metabolic Engineering, Departamento de Genética, Universidade Federal de Pernambuco, 50670-901, Recife, Brazil

      Marcos A. de Morais Jr.

    6. Department of Biology, University of Western Ontario, London, ON, N6A 5B7, Canada

      Marc-André Lachance

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    1. Letícia M. F. Sena
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    Correspondence to Carlos A. Rosa.

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    Letícia M. F. Sena and Camila G. Morais have contributed equally to this work.

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    Sena, L.M.F., Morais, C.G., Lopes, M.R. et al. d-Xylose fermentation, xylitol production and xylanase activities by seven new species of Sugiyamaella . Antonie van Leeuwenhoek 110, 53–67 (2017). https://doi.org/10.1007/s10482-016-0775-5

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