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Arenimonas alkanexedens sp. nov., isolated from a frozen soil sample

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Abstract

A novel facultatively anaerobic bacterium, designated strain LAM-WHM-D11T, was isolated from a frozen soil sample of China. Cells of strain LAM-WHM-D11T were observed to be Gram-stain negative, non-motile and rod-shaped. Colonies were yellowish, and circular with convex shape. Strain LAM-WHM-D11T was found to be able to grow at 4–40 °C (optimum 15 °C), pH 7.5–2.0 (optimum 9.5) and 0–2.5% NaCl (w/v) (optimum 1.5%). The 16S rRNA gene sequence similarity analysis showed that strain LAM-WHM-D11T is closely related to Arenimonas metalli CF5-1T (98.0%), Arenimonas aquaticum NA-09T (97.9%), Arenimonas donghaensis HO3-R19T (95.6%) and Arenimonas aestuarii S2-21T (95.3%). The DNA–DNA hybridization values between the isolate and A. metalli CGMCC 1.10787T, A. aquaticum KACC 14663T, A. donghaensis KACC 11381T were 41.0 ± 1.7, 44.7 ± 1.4 and 42.8 ± 1.2%, respectively. The genomic DNA G+C content was found to be 66.5 mol% as determined by the T m method. The major cellular fatty acids were identified as iso-C15:0 and iso-C16:0. The major isoprenoid quinone was identified as ubiquinone 8 (Q-8). The major polar lipids were found to be diphosphatidyglycerol, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylmonomethylethanolamine, two phospholipids and five unidentified lipids. Based on the phylogenetic, phenotypic and chemotaxonomic characteristics, strain LAM-WHM-D11T is concluded to represent a novel species within the genus Arenimonas, for which the name Arenimonas alkanexedens sp. nov. is proposed. The type strain is LAM-WHM-D11T (ACCC 19750T = JCM 30464T).

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Acknowledgements

This work was supported by Open Funds of Xinjiang Production and Construction Corps Key Laboratory of Protection and Utilization of Biological Resources in Tarim Basin (Tarim University, BRZD1603), Foundation of the Key Laboratory of Development and Application of Rural Renewable Energy (MOA, China) (No. 2015002) and National Infrastructure of Microbial Resources (NIMR-2017-1).

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Author notes

  1. Jie Zhu, Hui-Min Wang and Qi Zhang contributed equally to the work and share first authorship.

Authors and Affiliations

  1. College of Life Science, Xinjiang Normal University, Urumqi, 830046, XinJiang, China

    Jie Zhu, Qi Zhang & Wei Zhang

  2. Key Laboratory of Microbial Resources (Ministry of Agriculture, China), Institute of Agricultural Resources and Regional Planning, CAAS, Beijing, 100081, People’s Republic of China

    Jie Zhu, Hui-Min Wang, De-Long Kong, Xu Jiang & Zhi-Yong Ruan

  3. State Key Laboratory of Agricultural Microbiology, College of Life Science and Technology, Huazhong Agricultural University, Wuhan, 430070, China

    Wei-Wei Dong & Shu-Miao Zhao

  4. Key Laboratory of Development and Application of Rural Renewable Energy (MOA, China), Biogas Institute of Ministry of Agriculture, Chengdu, 610041, China

    Yan-Wei Wang

  5. Chinese Academy of Fishery Sciences, Beijing, 100141, China

    Jin-Long Song

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  1. Jie Zhu
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Correspondence to Wei Zhang or Zhi-Yong Ruan.

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Zhu, J., Wang, HM., Zhang, Q. et al. Arenimonas alkanexedens sp. nov., isolated from a frozen soil sample. Antonie van Leeuwenhoek 110, 1027–1034 (2017). https://doi.org/10.1007/s10482-017-0876-9

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