A full-length cDNA clone encoding the eukaryotic translation initiation factor 4A, isoform 2 (EIF4A2), was cloned from the fetal skeletal cDNA library from the pig (Sus scrofa). EIF4A2 is a highly conserved gene for one of the protein-synthesis initiation factors involved in the binding of mRNA to the ribosome. Based on this cDNA sequence, the deduced protein of 407 amino acids contains the characteristic motifs shared by the DEAD-box supergene family. The genomic nucleotide sequence of this gene was determined and a single nucleotide polymorphism located in the 5′ untranslated region was genotyped. The porcine EIF4A2 was expressed in all tissues examined but in variable amounts. The EIF4A2 expression level in muscle was upregulated through embryonic and neonatal development until adult, suggesting that porcine EIF4A2 was possibly involved in translation regulation of other muscle-related genes in muscle formation and development. In addition, we mapped porcine EIF4A2 to q4.1 of SSC13, in agreement with comparative mapping data.
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ACKNOWLEDGMENTS
We thank Dr. Martine Yerle of INRA, France, for kindly providing RH panels. This research was supported by the National Natural Science Foundation of China (30571330), Key Project of the National Natural Science Foundation of China (30330440), National 10th Five-Year Scientific Project of China for Tackling the Key Problems (2004BA717B), Key Project of the Scientific Research Foundation of the Ministry of Human Resources of China for Returned Chinese Scholars, State Platform of Technology Infrastructure of China (2004DKA30450), and National 973 and 863 Programs of China.
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Wang, H., Wang, H., Zhu, Z. et al. Molecular Cloning, Mapping, and Expression Analysis of the EIF4A2 Gene in Pig. Biochem Genet 45, 51–62 (2007). https://doi.org/10.1007/s10528-006-9065-7
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DOI: https://doi.org/10.1007/s10528-006-9065-7