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Expression of NAD+-dependent formate dehydrogenase in Enterobacter aerogenes and its involvement in anaerobic metabolism and H2 production

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Abstract

An expression system for NAD+-dependent formate dehydrogenase gene (fdh1), from Candida boidinii, was constructed and cloned into Enterobacter aerogenes IAM1183. With the fdh1 expression, the total H2 yield was attributed to a decrease in activity of the lactate pathway and an increase of the formate pathway flux due to the NADH regeneration. Analysis of the redox state balance and ethanol-to-acetate ratio in the fdhl-expressed strain showed that increased reducing power arose from the reconstruction of NADH regeneration pathway from formate thereby contributing to the improved H2 production.

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Acknowledgements

This work was supported in part by the National Basic Research Program of China (973 Plan) (Grant No. 2009CB724702), the Hi-Tech Research and Development Program of China (863 Plan) (Grant No. 2006AA02Z203) and the National Natural Science Foundation of China (Grant No. 20806046).

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Authors and Affiliations

  1. Institute of Biochemical Engineering, Department of Chemical Engineering, Tsinghua University, Beijing, 100084, China

    Yuan Lu, Hongxin Zhao, Chong Zhang, Qiheng Lai, Xi Wu & Xin-Hui Xing

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  1. Yuan Lu
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  2. Hongxin Zhao
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  3. Chong Zhang
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  4. Qiheng Lai
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  5. Xi Wu
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  6. Xin-Hui Xing
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Correspondence to Xin-Hui Xing.

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Lu, Y., Zhao, H., Zhang, C. et al. Expression of NAD+-dependent formate dehydrogenase in Enterobacter aerogenes and its involvement in anaerobic metabolism and H2 production. Biotechnol Lett 31, 1525–1530 (2009). https://doi.org/10.1007/s10529-009-0036-z

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  • DOI: https://doi.org/10.1007/s10529-009-0036-z

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