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Novel Escherichia coli hybrids with enhanced butanol tolerance

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Abstract

Hybrids between Escherichia coli and Lactobacillus brevis were generated via protoplast fusion. Growth kinetics of five hybrid strains and E. coli were used to evaluate the butanol tolerance of the novel strains under different conditions. The hybrid strains tolerated up to 2% (v/v) butanol compared to the 1% (v/v) maximum for E. coli. The growth inhibitory effects of butanol were also significantly less in several of the hybrids compared to E. coli. These results demonstrate the potential use of protoplast fusion to generate butanol-tolerant strains.

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Acknowledgements

We thank Dr. Albert Lee for assistance with experimentation and comments on the manuscript, Maria Priscilla Almario Falla for assistance with experimentation, and the National Science Foundation REU Grant 0552655 (MR) for funding.

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Author notes

  1. Matthew Rehmann

    Present address: Department of Chemical and Biomolecular Engineering, University of Pennsylvania, Philadelphia, PA, 19104, USA

Authors and Affiliations

  1. Department of Chemical Engineering, Texas A&M University, College Station, TX, 77843, USA

    James Winkler & Katy C. Kao

Authors
  1. James Winkler
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  2. Matthew Rehmann
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  3. Katy C. Kao
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Corresponding author

Correspondence to Katy C. Kao.

Additional information

James Winkler and Matthew Rehmann contributed equally to this work.

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Winkler, J., Rehmann, M. & Kao, K.C. Novel Escherichia coli hybrids with enhanced butanol tolerance. Biotechnol Lett 32, 915–920 (2010). https://doi.org/10.1007/s10529-010-0247-3

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  • DOI: https://doi.org/10.1007/s10529-010-0247-3

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